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Comparison of a competitive inhibition ELISA and the card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centrale in cattle

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Molloy, J.B., Bowles, P.M., Knowles, D.P., McElwain, T.F., Bock, R.E., Kingston, T.G., Blight, G.W. and Dalgliesh, R.J. (1999) Comparison of a competitive inhibition ELISA and the card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centrale in cattle. Australian Veterinary Journal, 77 (4). pp. 245-249. ISSN 0005-0423

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Article Link: https://doi.org/10.1111/j.1751-0813.1999.tb11712.x

Abstract

Objective: To compare a recently developed recombinant MSP-5 competitive inhibition ELISA with a card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centralein Australian cattle.

Materials and methods: The ELISA was compared with the card agglutination test using 208 sera from cattle in Anaplasma-free herds, 86 sera from cattle experimentally infected with A marginale or A centrale and 757 sera from cattle in areas endemic for A marginale.

Results: The specificity of the ELISA, based on testing 208 sera from cattle in Anaplasma-free areas, was 99.5%, and the sensitivities for detection of antibodies to A marginale and A centrale in sera from the experimentally infected cattle were 98.0% and 100%, respectively. For the same sets of sera, the specificity of the card agglutination test was 98.6% and the sensitivities for detection of antibodies to A marginale and A centrale were 98.0% and 100%, respectively. For the 757 sera collected from cattle in areas endemic for A marginale, the agreement between the ELISA and the card agglutination test depended on the positive threshold selected for the ELISA. The maximum achievable agreement was 91.5% (kappa = 0.73; 95% confidence interval 0.66, 0.79).

Conclusion: We conclude that the competitive inhibition ELISA is a useful alternative to the card agglutination test for detection of A marginale or A centrale infection in cattle. The assay should be particularly useful for epidemiological applications such as prevalence studies and control programs.

Item Type:Article
Subjects:Veterinary medicine > Veterinary epidemiology. Epizootiology
Veterinary medicine > Diseases of special classes of animals > Cattle
Live Archive:06 Mar 2024 00:48
Last Modified:06 Mar 2024 00:48

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