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In vitro propagation of banana (Musa spp.): initiation, proliferation and development of shoot-tip cultures on defined media

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Wong, W.C. (1986) In vitro propagation of banana (Musa spp.): initiation, proliferation and development of shoot-tip cultures on defined media. Plant Cell, Tissue and Organ Culture (PCTOC), 6 . pp. 159-166. ISSN 0167-6857

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Article Link: https://doi.org/10.1007/BF00180799

Abstract

In vitro multiplication of banana (Musa spp.) from shoot-tip explants isolated from lateral suckers is described. Using explants with apical domes, a total of 22 banana cultivars were successfully cultured on a modified Murashige and Skoog's medium containing 6-benzylaminopurine (BA) and indolebutyric acid (IBA). Shoot-tip explants could be induced to produce multiple shoot initials in the presense or absence of apical domes, but the survival rates were higher if apical domes were retained. Cultivars varied widely in their multiplication rates in response to cytokinins, BA being consistently more effective than kinetin (Kn). Although Kn was less effective in this regard, it stimulated vigorous root growth. Rooted plantlets were successfully established in soil.

Item Type:Article
Subjects:Plant culture > Propagation
Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Bananas
Live Archive:16 Feb 2024 01:10
Last Modified:16 Feb 2024 01:10

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