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Validation of analytical parameters of a competitive direct ELISA for aflatoxin B1 in peanuts

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Lee, N.A., Rachaputi, R. C. N., Wright, G. C., Krosch, S., Norman, K., Anderson, J., Ambarwati, S., Retnowati, I., Dharmaputra, O.S. and Kennedy, I.R. (2005) Validation of analytical parameters of a competitive direct ELISA for aflatoxin B1 in peanuts. Food and Agricultural Immunology, 16 (2). pp. 149-163. ISSN 0954-0105


Article Link: https://doi.org/10.1080/09540100500159179


Analytical validation of a competitive direct SUNQuik ELISA with a reference High Performance Liquid Chromatography (HPLC) method and other methods including a minicolumn method and the VICAM Aflatest® system for aflatoxin in peanuts was conducted. Both the ELISA and the VICAM Aflatest® system, using the same peanut extracts were analytically comparable with the HPLC method (R=0.998, p<0.000). The minicolumn method was also found to be acceptable as a low cost rapid semi-quantitative test. Despite the large variation in sampling, the correlation between the SUNQuik ELISA and HPLC using the different peanut sub-samples was considered acceptable over the range of 0–1200 µg kg−1 (R=0.938). No false negatives were found using the SUNQuik ELISA and false positives were either nil or negligible in all the studies conducted. The repeatability of the SUNQuik ELISA run on the same day was good with only±10% deviation. The reproducibility of the SUNQuik ELISA between days was also acceptable, but with a higher deviation. Applying the SUNQuik ELISA for aflatoxin surveys of peanuts in Indonesia proved that the method can deliver high quality, cost- and time-effective analysis with very little establishment capital and maintenance.

Item Type:Article
Subjects:Plant culture > Food crops
Live Archive:05 Feb 2024 23:05
Last Modified:05 Feb 2024 23:05

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