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Transpecific microsatellites for hard pines

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Shepherd, M., Cross, M., Maguire, T., Dieters, M., Williams, C. and Henry, R. (2002) Transpecific microsatellites for hard pines. Theoretical and Applied Genetics, 104 . pp. 819-827. ISSN 0040-5752

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Article Link: https://doi.org/10.1007/s00122-001-0794-z


Microsatellites are difficult to recover from large plant genomes so cross-specific utilisation is an important source of markers. Fifty microsatellites were tested for cross-specific amplification and polymorphism to two New World hard pine species, slash pine (Pinus elliottii var. elliottii) and Caribbean pine (P. caribaea var. hondurensis). Twenty-nine (58%) markers amplified in both hard pine species, and 23 of these 29 were polymorphic. Soft pine (subgenus Strobus) microsatellite markers did amplify, but none were polymorphic. Pinus elliottii var. elliottii and P. caribaea var. hondurensis showed mutational changes in the flanking regions and the repeat motif that were informative for Pinus spp. phylogenetic relationships. Most allele length variation could be attributed to variability in repeat unit number. There was no evidence for ascertainment bias.

Item Type:Article
Subjects:Science > Botany > Genetics
Forestry > Research. Experimentation
Live Archive:18 Jan 2024 00:32
Last Modified:18 Jan 2024 00:32

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