Login | Request Account (DAF staff only)

Hemolymph clotting time as an indicator of stress in western rock lobster (Panulirus cygnus George)

Share this record

Add to FacebookAdd to LinkedinAdd to XAdd to WechatAdd to Microsoft_teamsAdd to WhatsappAdd to Any

Export this record

View Altmetrics

Jussila, J., McBride, S., Jago, J. and Evans, L.H. (2001) Hemolymph clotting time as an indicator of stress in western rock lobster (Panulirus cygnus George). Aquaculture, 199 (1-2). pp. 185-193. ISSN 0044-8486

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link: https://doi.org/10.1016/S0044-8486(00)00599-8

Abstract

A simple method to measure clotting time was introduced in an experiment studying the effects of exercise stress on western rock lobster (Panulirus cygnus). The treatment group lobsters (N=8) were made to exercise (tail flipping) in a 200 l tank for 2 min. Lobsters were then sampled immediately after exercise and once again 1 h later. Controls were sampled both before the experiment started and after all the other samples were taken (pre- and post-exercise controls). Total hemocyte counts (THCs), differential hemocyte counts (DHCs), hemolymph clotting time, hemolymph protein content and lobster vigor were analyzed. THCs, DHCs, hemolymph clotting and vigor index showed response to treatment. THCs were increased significantly 1 h after exercise (6.36±0.58×106 cells/ml) compared to pre- and post-exercise controls (4.42±0.38 and 4.41±0.38×106 cells/ml, respectively). Hemolymph clotting time in the same experimental lobsters (43.7±2.0 s) were decreased compared to pre- and post-exercise control groups (56.0±4.1 and 52.7±2.2 s, respectively). Hemolymph clotting times were correlating with DHCs and hemolymph protein levels. Hemolymph clotting time is easily measured and could be a useful indicator of stress.

Item Type:Article
Subjects:Aquaculture and Fisheries > Fisheries > Fishery for individual species
Live Archive:09 Jan 2024 01:07
Last Modified:09 Jan 2024 01:07

Repository Staff Only: item control page