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Assessment and rationalization of genetic diversity of Papua New Guinea taro (Colocasia esculenta) using SSR DNA fingerprinting

Singh, D. and Mace, E.S and Godwin, I.D. and Mathur, P.N. and Okpul, T. and Taylor, M. and Hunter, D. and Kambuou, R. and Rao, V.R. and Jackson, G. (2007) Assessment and rationalization of genetic diversity of Papua New Guinea taro (Colocasia esculenta) using SSR DNA fingerprinting. Genetic Resources and Crop Evolution, 55 (6). pp. 811-822.

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Article Link(s): http://dx.doi.org/10.1007/s10722-007-9286-6

Publisher URL: http://www.springerlink.com

Abstract

Taro (Colocasia esculenta) accessions were collected from 15 provinces of Papua New Guinea (PNG). The collection, totalling 859 accessions was collated for characterization and a core collection of 81 accessions (10%) was established on the basis of characterization data generated on 30 agro-morphological descriptors, and DNA fingerprinting using seven SSR primers. The selection of accessions was based on cluster analysis of the morphological data enabling initial selection of 20% accessions. The 20% sample was then reduced and rationalized to 10% based on molecular data generated by SSR primers. This represents the first national core collection of any species established in PNG based on molecular markers. The core has been integrated with core from other Pacific Island countries, contributing to a Pacific regional core collection, which is conserved in vitro in the South Pacific Regional Germplasm Centre at Fiji. The core collection is a valuable resource for food security of the South Pacific region and is currently being utilized by the breeding programmes of small Pacific Island countries to broaden the genetic base of the crop.

Item Type:Article
Corporate Creators:Department of Employment, Economic Development and Innovation (DEEDI), Agri-Science, Crop and Food Science, Plant Science
Business groups:Agri-Science, Crop and Food Science
Additional Information:© Springer Science+Business Media B.V.
Keywords:Agro-morphology; characterisation; Colocasia esculenta; core collection; DNA fingerprinting; simple sequence repeats (SSR); taro; Papua New Guinea.
Subjects:Plant culture > Propagation
Science > Biology > Genetics
Agriculture > Agriculture (General) > Documents and other collections
Deposited On:12 Jan 2009 04:49
Last Modified:26 Oct 2011 02:07

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