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Single-strand restriction fragment length polymorphism analysis of the second internal transcribed spacer (ribosomal DNA) for six species of Eimeria from chickens in Australia

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Woods, W.G., Whithear, K.G., Richards, D.G., Anderson, G. R., Jorgensen, W.K. and Gasser, R.B. (2000) Single-strand restriction fragment length polymorphism analysis of the second internal transcribed spacer (ribosomal DNA) for six species of Eimeria from chickens in Australia. International Journal for Parasitology, 30 (9). pp. 1019-1023. ISSN 0020-7519

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Article Link: https://doi.org/10.1016/S0020-7519(00)00084-9

Abstract

Species of Eimeria from chickens from Australia were characterised using a polymerase chain reaction-linked restriction fragment length polymorphism (PCR-RFLP) approach. The ribosomal DNA region spanning the second internal transcribed spacer (ITS-2) was amplified from genomic DNA by PCR, digested separately with three restriction endonucleases (CfoI, Sau3AI and TaqI) and the fragments separated by denaturing gel electrophoresis. The PCR products amplified from the six species varied from ∼70 to 620 bp on agarose gels, with differences in size and number of bands among species, but no apparent variation within a species. The PCR-RFLP analysis of ITS-2 amplicons on denaturing gels gave characteristic profiles for individual species (except for minor variation in profiles within some species). The results indicate that ITS-2 contains useful genetic markers for the identification of six Eimeria species occurring in Australia.

Item Type:Article
Subjects:Veterinary medicine > Veterinary parasitology
Veterinary medicine > Diseases of special classes of animals > Avian
Live Archive:08 Jan 2024 00:26
Last Modified:08 Jan 2024 00:26

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