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Association of 'Candidatus Phytoplasma australiense' with green petal and lethal yellows diseases in strawberry

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Padovan, A., Gibb, K. S. and Persley, D. M. (2000) Association of 'Candidatus Phytoplasma australiense' with green petal and lethal yellows diseases in strawberry. Plant Pathology, 49 (3). pp. 362-369. ISSN 0032-0862

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Article Link: https://doi.org/10.1046/j.1365-3059.2000.00461.x

Abstract

The identity of phytoplasmas detected in strawberry plants with green petal (SGP) and lethal yellows (SLY) diseases was determined by RFLP analysis of the 16S rRNA gene and adjacent spacer region (SR). RFLP and sequence comparisons indicated that the phytoplasmas associated with SGP and SLY were indistinguishable and were most closely related to ‘Candidatus Phytoplasma australiense’, the phytoplasma associated with Australian grapevine yellows, papaya dieback and Phormium yellow leaf diseases. This taxon lies within the aster yellows strain cluster. Primers based on the phytoplasma tuf gene, which amplify only members of the AY strain cluster, amplified a DNA product from the SGP and SLY phytoplasmas. Primers deduced from the 16S rRNA/SR of P. australiense that amplify only members of this taxon amplified rDNA sequences from the SGP and SLY phytoplasmas. Primers that selectively amplify members of the faba bean phyllody (FBP) phytoplasma group, the most commonly occurring phytoplasma group in Australia, did not amplify rDNA from the SGP and SLY phytoplasmas.

Item Type:Article
Subjects:Plant pests and diseases > Plant pathology
Live Archive:05 Jan 2024 05:39
Last Modified:05 Jan 2024 05:39

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