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Characterisation of Australian isolates of Fusarium oxysporum f. sp cubense by DNA fingerprinting analysis

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Gerlach, K.S., Bentley, S., Moore, N. Y., Pegg, K.G. and Aitken, E. A. B. (2000) Characterisation of Australian isolates of Fusarium oxysporum f. sp cubense by DNA fingerprinting analysis. Australian Journal of Agricultural Research, 51 (8). pp. 945-953. ISSN 1836-0947


Article Link: https://doi.org/10.1071/AR99172


Genetic variation among Australian isolates of the fungus Fusarium oxysporum f. sp. cubense (Foc), which causes Fusarium wilt in banana, was examined using DNA amplification fingerprinting (DAF). Ninety-four isolates which represented Races 1, 2, 3, and 4, and vegetative compatibility groups (VCGs) 0120, 0124, 0125, 0128, 0129, 01211, 01213/16, and 01220 were analysed. The genetic relatedness among isolates within each VCG, and between the 8 different VCGs of Foc present in Australia was determined. The DNA fingerprint patterns were VCG-specific, with each VCG representing a unique genotype. The genetic similarity among isolates within each VCG ranged from 97% to 100%. Among the different VCGs of Foc, 3 major clusters were distinguished which corresponded with race. All Race 1 and 2 isolates (VCGs 0124, 0125, 0128, and 01220) were closely related and clustered together, the Race 3 isolates from Heliconia clustered separately, and all Race 4 isolates (VCGs 0120, 0129, 01211, and 01213/16) clustered together.
Fifteen isolates from Alstonville, NSW, were characterised because although they were classified as Race 2 based on their recovery from cooking banana cultivars, they belonged in VCG 0124, which had previously contained only Race 1 isolates. The occurrence of more than one race within a VCG means that vegetative compatibility grouping cannot be used to assign pathotype to pathogenic race as previously thought. It was possible to distinguish the Race 1 and Race 2 isolates within VCG 0124 using DNA fingerprinting, as each race produced a unique DNA fingerprint pattern. Among the Australian isolates, DNA fingerprinting analysis identified 9 different VCGs and genotypes of Foc.

Item Type:Article
Subjects:Plant pests and diseases > Individual or types of plants or trees > Bananas
Live Archive:04 Jan 2024 22:18
Last Modified:10 Jan 2024 00:09

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