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Genetic transformation of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment

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Becker, D. K., Dugdale, B., Smith, M. K., Harding, R. M. and Dale, J. L. (2000) Genetic transformation of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment. Plant Cell Reports, 19 . pp. 229-234. ISSN 1432-203X

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Article Link: https://doi.org/10.1007/s002990050004

Abstract

An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotransferase (nptII) selectable marker gene under the control of a banana bunchy top virus (BBTV) promoter or the CaMV 35S promoter, and either the β-glucuronidase (uidA) reporter gene or BBTV genes under the control of the maize polyubiquitin promoter. Plants were regenerated, under selection with kanamycin, that were co-transformed with nptII and either the uidA or BBTV genes. Molecular characterisation of transformants demonstrated that the transgenes had been stably integrated into the banana genome.

Item Type:Article
Corporate Creators:Department of Primary Industries, Queensland
Subjects:Science > Botany > Genetics
Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Bananas
Live Archive:03 Jan 2024 02:31
Last Modified:03 Jan 2024 02:31

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