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Molecular techniques for monitoring bacterial and bacteriophage populations in the rumen

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Attwood, G. T. and Klieve, A. V. (2000) Molecular techniques for monitoring bacterial and bacteriophage populations in the rumen. In: Microbial Biosystems: New Frontiers. Proceedings of the 8th International Symposium on Microbial Ecology (1998). Atlantic Canadian Society for Microbial Ecology, Canada. ISBN 9780968676332



Molecular techniques are increasingly being used in microbial ecology to identify microorganisms, infer phylogenetic relationships, and describe community structure. We have used rRNA sequence information to develop a competitive PCR technique which enumerates proteolytic bacterial populations from the forestomach of pasture-grazed ruminants. PCR primer pairs, which circumscribe several proteolytic rumen bacterial populations, were used in co-amplifications of target and internal control DNAs, to quantify individual microbial populations. The results from enumerations in pasture-fed animals will be discussed in relation to the sensitivity, specificity and quantitative aspects of the technique. Rumen bacteriophage also impact significantly on rumen metabolism and are being quantified by DNA-based techniques. Studies using pulsed-field gel electrophoresis and laser densitometry of purified phage DNA have shown large variations in ruminal bacteriophage numbers and bacteriophage types. These molecular techniques provide better descriptions of individual ruminal populations and eventually will allow for a complete description of the rumen ecosystem.

Item Type:Book Section
Corporate Creators:Department of Primary Industry, Queensland
Subjects:Veterinary medicine > Veterinary microbiology
Veterinary medicine > Veterinary medicine of special organs, regions and systems
Live Archive:03 Jan 2024 01:24
Last Modified:03 Jan 2024 02:14

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