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Gamma irradiation of dried plant material: implications for the identification of plant pathogens employing molecular techniques

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Jones, L.M., Grice, K. R.E. and Davis, R.I. (2010) Gamma irradiation of dried plant material: implications for the identification of plant pathogens employing molecular techniques. Australasian Plant Pathology, 39 (4). pp. 358-362. ISSN 0815-3191

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Article Link: https://doi.org/10.1071/ap09093

Abstract

Five pathogen-host leaf tissue combinations were used to determine whether dried plant disease material gamma (g) irradiated at a level of 25 kGy retains diagnostic value when subjected to selected polymerase chain reactionbased assays. Diagnostic amplification of nucleic acids from all anticipated positive samples was successful following g irradiation. Variability in results is discussed in relation to sample handling.

Item Type:Article
Business groups:Horticulture and Forestry Science
Subjects:Plant pests and diseases > Plant pathology
Live Archive:16 Feb 2023 06:42
Last Modified:16 Feb 2023 06:42

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