Login | Request Account (DAF staff only)

The in planta gene expression of Austropuccinia psidii in resistant and susceptible Eucalyptus grandis

Share this record

Add to FacebookAdd to LinkedinAdd to XAdd to WechatAdd to Microsoft_teamsAdd to WhatsappAdd to Any

Export this record

View Altmetrics

Swanepoel, S., Visser, E. A., Shuey, L. S. and Naidoo, S. (2023) The in planta gene expression of Austropuccinia psidii in resistant and susceptible Eucalyptus grandis. Phytopathology® (ja). null.

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link: https://doi.org/10.1094/PHYTO-07-22-0257-R

Publisher URL: https://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-07-22-0257-R


Austropuccinia psidii, commonly known as myrtle rust, is an obligate, biotrophic rust pathogen that causes rust disease on a broad host range of Myrtaceae species. Eucalyptus grandis, a widely cultivated hardwood Myrtaceae species, is susceptible to A. psidii infection, with this pathogen threatening both their natural range and various forest plantations across the world. This study aimed to investigate the A. psidii transcriptomic responses in resistant and susceptible E. grandis at four time points. RNA-seq reads were mapped to the A. psidii reference genome to quantify expressed genes at 12-hours post inoculation (hpi), 1-, 2- and 5-days post inoculation (dpi). A total of eight hundred and ninety expressed genes were found, of which forty-three were candidate effector proteins. These included a rust transferred protein (RTP1) gene, expressed in susceptible hosts at 5-dpi and a hydrolase protein gene expressed in both resistant and susceptible hosts over time. Functional categorisation of expressed genes revealed processes enriched in susceptible hosts, including malate metabolic and malate dehydrogenase activity, implicating oxalic acid in disease susceptibility. These results highlight putative virulence or pathogenicity mechanisms employed by A. psidii to cause disease and provides the first insight into the molecular responses of A. psidii in E. grandis over time.

Item Type:Article
Business groups:Horticulture and Forestry Science
Keywords:Bioinformatics,Fungal Pathogens,Genomics,Host Parasite Interactions,Pathogen Effectors
Subjects:Plant pests and diseases
Plant pests and diseases > Plant pathology
Forestry > Research. Experimentation
Live Archive:13 Jan 2023 06:11
Last Modified:13 Jan 2023 06:11

Repository Staff Only: item control page