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Development of a Novel Tissue Blot Hybridization Chain Reaction for the Identification of Plant Viruses

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Filardo, F. F., Vukovic, P., Sharman, M., Gambley, C. and Campbell, P. R. (2022) Development of a Novel Tissue Blot Hybridization Chain Reaction for the Identification of Plant Viruses. Plants (Basel), 11 (17). p. 2325.

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Article Link(s): https://doi.org/10.3390/plants11172325

Abstract

Assays for the high throughput screening of crops for virus monitoring need to be quick, easy, and low cost. One method involves using tissue blot immunoassays (TBIA), where plant stems are blotted onto nitrocellulose membrane and screened with available antibodies against a range of viruses. TBIAs are inexpensive but limited by antibody availability and specificity. To circumvent the antibody limitations, we developed the tissue blot hybridization chain reaction (TB-HCR). As with TBIA, plant stems are blotted onto a nitrocellulose membrane, however, TB-HCR involves using nucleic acid probes instead of antibodies. We demonstrated for the first time that TB-HCR can be used for plant viruses by designing and testing probes against species from several virus genera including begomovirus, polerovirus, luteovirus, cucumovirus, and alfamovirus. We also explored different hairpin reporter methods such as biotin/streptavidin-AP and the Alexa Fluor-488 Fluorophore. TB-HCR has applications for low-cost diagnostics for large sample numbers, rapid diagnostic deployment for new viruses, and can be performed as a preliminary triage assay prior to downstream applications.

Item Type:Article
Business groups:Horticulture and Forestry Science, Crop and Food Science
Keywords:Amv Cmv Tylcv hybridization chain reaction luteoviruses plant viruses polerovirus self-assembly tissue blot
Subjects:Plant pests and diseases
Plant pests and diseases > Plant pathology
Deposited On:24 Nov 2022 01:05
Last Modified:24 Nov 2022 01:05

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