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Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle

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Goff, W.L., Johnson, W.C., Molloy, J.B., Jorgensen, W.K., Waldron, S.J., Figueroa, J.V., Matthee, O., Adams, D.S., McGuire, T.C., Pino, I., Mosqueda, J., Palmer, G.H., Suarez, C.E., Knowles, D.P. and McElwain, T.F. (2008) Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle. Clinical and Vaccine Immunology, 15 (9). pp. 1316-1321.

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Article Link: http://dx.doi.org/10.1128/CVI.00150-08

Publisher URL: http://journals.asm.org


A competitive enzyme-linked immunosorbent assay (cELISA) based on a broadly conserved, species-specific, B-cell epitope within the C terminus of Babesia bigemina rhoptry-associated protein 1a was validated for international use. Receiver operating characteristic analysis revealed 16% inhibition as the threshold for a negative result, with an associated specificity of 98.3% and sensitivity of 94.7%. Increasing the threshold to 21% increased the specificity to 100% but modestly decreased the sensitivity to 87.2%. By using 21% inhibition, the positive predictive values ranged from 90.7% (10% prevalence) to 100% (95% prevalence) and the negative predictive values ranged from 97.0% (10% prevalence) to 48.2% (95% prevalence). The assay was able to detect serum antibody as early as 7 days after intravenous inoculation. The cELISA was distributed to five different laboratories along with a reference set of 100 defined bovine serum samples, including known positive, known negative, and field samples. The pairwise concordance among the five laboratories ranged from 100% to 97%, and all kappa values were above 0.8, indicating a high degree of reliability. Overall, the cELISA appears to have the attributes necessary for international application.

Item Type:Article
Corporate Creators:Emerging Technologies
Additional Information:© American Society for Microbiology.
Keywords:Rhoptry-associated protein 1a; expression; epitopes; Bovis; infection; surface; genes.
Subjects:Science > Science (General)
Veterinary medicine > Veterinary parasitology
Veterinary medicine > Diseases of special classes of animals > Cattle
Live Archive:02 Feb 2009 02:02
Last Modified:03 Sep 2021 16:47

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