Login | Request Account (DAF staff only)

Tomato yellow leaf curl virus in Australia: distribution, detection and discovery of naturally occurring defective DNA molecules

View Altmetrics

Van Brunschot, S. L., Persley, D. M., Geering, A. D. W., Campbell, P. R. and Thomas, J. E. (2010) Tomato yellow leaf curl virus in Australia: distribution, detection and discovery of naturally occurring defective DNA molecules. Australasian Plant Pathology, 39 (5). pp. 412-423. ISSN 1448-6032

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link(s): https://doi.org/10.1071/AP10083

Abstract

Tomato yellow leaf curl virus (TYLCV) was detected for the first time in Australia in March 2006 in field-grown tomatoes in Brisbane, Queensland. Surveys showed that the virus was confined to south-east Queensland. Virus transmission studies carried out using Bemisia tabaci (B biotype) verified that resistant tomato lines containing the Ty-1 or Ty-5 genes displayed tolerance to infection by TYLCV isolates from Australia. A PCR assay specific for TYLCV was designed and optimised to confirm the presence of the virus in samples that tested positive in begomovirus-specific double-antibody sandwich enzyme-linked immunosorbent assay. Eight isolates of TYLCV from various sites were cloned and sequenced, and were shown to have near-identical sequences and a high nucleotide sequence similarity (98%) to the monopartite Tomato yellow leaf curl virus-Israel (TYLCV-IL). No DNA-B, DNA-1 nor DNA-b satellite molecules were detected using degenerate PCR assays. Phylogenetic analysis revealed that Australian isolates of TYLCV separated into two sequence groups, TYLCV-IL[Au:Bri:06] and TYLCV-IL[Au:Bun:06], that showed a defined geographic segregation. Naturally occurring defective DNA molecules containing partial, rearranged segments of the native DNA-A, were present in one isolate. To our knowledge, this is the first report of an incursion of a begomovirus into Australia, and the first report of the characterisation of naturally occurring defective DNAs of TYLCV. Additional keywords: diagnostics, Geminiviridae, rolling-circle amplification, virus host range.

Item Type:Article
Business groups:Horticulture and Forestry Science
Keywords:Australasian Plant Pathology Tomato Yellow Leaf Curl Virus Tomato Yellow Leaf African Cassava Mosaic Virus Monopartite Begomoviruses
Subjects:Plant culture > Vegetables
Plant culture > Fruit and fruit culture
Plant pests and diseases
Plant pests and diseases > Plant pathology
Deposited On:12 Apr 2022 01:45
Last Modified:12 Apr 2022 01:45

Repository Staff Only: item control page