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Comparative immunogenicity of M. hyopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S. typhimurium aroA in mice.

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Chen, A.Y., Fry, S.R., Forbes-Faulkner, J., Daggard, G.E. and Mukkur, T.K.S. (2006) Comparative immunogenicity of M. hyopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S. typhimurium aroA in mice. Veterinary Microbiology, 114 (3-4). pp. 252-259.

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Article Link: http://dx.doi.org/10.1016/j.vetmic.2005.12.009

Publisher URL: http://www.elsevier.com

Abstract

The Mycoplasma hyopneumoniae ribonucleotide reductase R2 subunit (NrdF) gene fragment was cloned into eukaryotic and prokaryotic expression vectors and its immunogenicity evaluated in mice immunized orally with attenuated Salmonella typhimurium aroA CS332 harboring either of the recombinant expression plasmids. We found that NrdF is highly conserved among M. hyopneumoniae strains. The immunogenicity of NrdF was examined by analyzing antibody responses in sera and lung washes, and the cell-mediated immune (CMI) response was assessed by determining the INF-[gamma] level produced by splenocytes upon in vitro stimulation with NrdF antigen. S. typhimurium expressing NrdF encoded by the prokaryotic expression plasmid (pTrcNrdF) failed to elicit an NrdF-specific serum or secretory antibody response, and IFN-[gamma] was not produced. Similarly, S. typhimurium carrying the eukaryotic recombinant plasmid encoding NrdF (pcNrdF) did not induce a serum or secretory antibody response, but did elicit significant NrdF-specific IFN-[gamma] production, indicating induction of a CMI response. However, analysis of immune responses against the live vector S. typhimurium aroA CS332 showed a serum IgG response but no mucosal IgA response in spite of its efficient invasiveness in vitro. In the present study we show that the DNA vaccine encoding the M. hyopneumoniae antigen delivered orally via a live attenuated S. typhimurium aroA can induce a cell-mediated immune response. We also indicate that different live bacterial vaccine carriers may have an influence on the type of the immune response induced.

Item Type:Article
Corporate Creators:Biosecurity Queensland
Additional Information:© Elsevier.
Keywords:Mycoplasma hyopneumoniae; NrdF antigen; oral immunization; immunogenicity.
Subjects:Veterinary medicine > Diseases of special classes of animals > Swine
Veterinary medicine > Veterinary microbiology
Live Archive:04 Nov 2008 01:12
Last Modified:03 Sep 2021 16:47

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