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Saprophytic microorganisms with potential for biological control of Botrytis cinerea on Geraldton waxflower flowers

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Beasley, D. R., Joyce, D. C., Coates, L. M. and Wearing, A. H. (2001) Saprophytic microorganisms with potential for biological control of Botrytis cinerea on Geraldton waxflower flowers. Australian Journal of Experimental Agriculture, 41 (5). pp. 697-703. ISSN 08161089 (ISSN)


Article Link: https://doi.org/10.1071/EA00112


Saprophytic bacteria, yeasts and filamentous fungi were isolated from Geraldton waxflower flowers and screened to identify potential antagonism towards Botrytis cinerea. Isolates from other sources (e.g. avocado) were also tested. Isolates were initially screened in vitro for inhibition of B. cinerea conidial germination, germ tube elongation and mycelial growth. The most antagonistic bacteria, yeasts and fungi were selected for further testing on detached waxflower flowers. Conidia of the pathogen were mixed with conidia or cells of the selected antagonists, co-inoculated onto waxflower flowers, and the flowers were sealed in glass jars and incubated at 20°C. The number of days required for the pathogen to cause flower abscission was determined. The most antagonistic bacterial isolate, Pseudomonas sp. 677, significantly reduced conidial germination and retarded germ tube elongation of B. cinerea. None of the yeast or fungal isolates tested was found to significantly reduce conidial germination or retard germ tube elongation, but several significantly inhibited growth of B. cinerea. Fusarium sp., Epicoccum sp. and Trichoderma spp. were the most antagonistic of these isolates. Of the isolates tested on waxflower, Pseudomonas sp. 677 was highly antagonistic towards B. cinerea and delayed waxflower abscission by about 3 days. Trichoderma harzianum also significantly delayed flower abscission. However, as with most of the fungal antagonists used, inoculation of waxflower flowers with this isolate resulted in unsightly mycelial growth.

Item Type:Article
Additional Information:Reproduced with permission from © CSIRO Publishing. Access to published version is available via Publisher’s website.
Keywords:abscission biocontrol agent biological pest control conidium culture vessel day length flower fungal infection genetic screening genetic strain inoculation plant fungus interaction spore germination antagonism biological control microorganism Bacteria (microorganisms) Botryotinia fuckeliana Botrytis Epicoccum Etlingera Fungi Fusarium Fusarium sp. Hypocrea lixii Persea americana Pseudomonas Pseudomonas sp. Trichoderma
Subjects:Plant culture > Flowers and flower culture. Ornamental plants
Plant pests and diseases
Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Organic plant protection. Biological control
Live Archive:28 Oct 2021 02:20
Last Modified:28 Oct 2021 02:20

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