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Genome-Wide Identification and Characterization of the Brassinazole-resistant (BZR) Gene Family and Its Expression in the Various Developmental Stage and Stress Conditions in Wheat (Triticum aestivum L.)

Kesawat, M. S., Kherawat, B. S., Singh, A., Dey, P., Kabi, M., Debnath, D., Saha, D., Khandual, A., Rout, S., Ali, A., Palem, R. R., Gupta, R., Kadam, A. A., Kim, H.-U., Chung, S.-M. and Kumar, M. (2021) Genome-Wide Identification and Characterization of the Brassinazole-resistant (BZR) Gene Family and Its Expression in the Various Developmental Stage and Stress Conditions in Wheat (Triticum aestivum L.). International Journal of Molecular Sciences, 22 (16). ISSN 1422-0067

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Article Link(s): https://doi.org/10.3390/ijms22168743

Abstract

Brassinosteroids (BRs) play crucial roles in various biological processes, including plant developmental processes and response to diverse biotic and abiotic stresses. However, no information is currently available about this gene family in wheat (Triticum aestivum L.). In the present investigation, we identified the BZR gene family in wheat to understand the evolution and their role in diverse developmental processes and under different stress conditions. In this study, we performed the genome-wide analysis of the BZR gene family in the bread wheat and identified 20 TaBZR genes through a homology search and further characterized them to understand their structure, function, and distribution across various tissues. Phylogenetic analyses lead to the classification of TaBZR genes into five different groups or subfamilies, providing evidence of evolutionary relationship with Arabidopsis thaliana, Zea mays, Glycine max, and Oryza sativa. A gene exon/intron structure analysis showed a distinct evolutionary path and predicted the possible gene duplication events. Further, the physical and biochemical properties, conserved motifs, chromosomal, subcellular localization, and cis-acting regulatory elements were also examined using various computational approaches. In addition, an analysis of public RNA-seq data also shows that TaBZR genes may be involved in diverse developmental processes and stress tolerance mechanisms. Moreover, qRT-PCR results also showed similar expression with slight variation. Collectively, these results suggest that TaBZR genes might play an important role in plant developmental processes and various stress conditions. Therefore, this work provides valuable information for further elucidate the precise role of BZR family members in wheat.

Item Type:Article
Business groups:Horticulture and Forestry Science
Additional Information:Open Access
Keywords:brassinosteroid qRT-PCR cis-acting regulatory elements biotic and abiotic stress
Subjects:Science > Botany > Genetics
Plant culture > Field crops > Wheat
Plant pests and diseases
Deposited On:01 Sep 2021 06:22
Last Modified:03 Sep 2021 16:46

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