Login | Request Account (DAF staff only)

Resistance and susceptibility QTL identified in a rice MAGIC population by screening with a minor-effect virulence factor from Xanthomonas oryzae pv. oryzae

View Altmetrics

Huerta, A. I., Delorean, E. E., Bossa-Castro, A. M., Tonnessen, B. W., Raghavan, C., Corral, R., Pérez-Quintero, Á. L., Leung, H., Verdier, V. and Leach, J. E. (2021) Resistance and susceptibility QTL identified in a rice MAGIC population by screening with a minor-effect virulence factor from Xanthomonas oryzae pv. oryzae. Plant Biotechnology Journal, 19 (1). pp. 51-63. ISSN 1467-7644

[img]
Preview
PDF
4MB

Article Link(s): https://doi.org/10.1111/pbi.13438

Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13438

Abstract

Summary Effective and durable disease resistance for bacterial blight (BB) of rice is a continuous challenge due to the evolution and adaptation of the pathogen, Xanthomonas oryzae pv. oryzae (Xoo), on cultivated rice varieties. Fundamental to this pathogens’ virulence is transcription activator-like (TAL) effectors that activate transcription of host genes and contribute differently to pathogen virulence, fitness or both. Host plant resistance is predicted to be more durable if directed at strategic virulence factors that impact both pathogen virulence and fitness. We characterized Tal7b, a minor-effect virulence factor that contributes incrementally to pathogen virulence in rice, is a fitness factor to the pathogen and is widely present in geographically diverse strains of Xoo. To identify sources of resistance to this conserved effector, we used a highly virulent strain carrying a plasmid borne copy of Tal7b to screen an indica multi-parent advanced generation inter-cross (MAGIC) population. Of 18 QTL revealed by genome-wide association studies and interval mapping analysis, six were specific to Tal7b (qBB-tal7b). Overall, 150 predicted Tal7b gene targets overlapped with qBB-tal7b QTL. Of these, 21 showed polymorphisms in the predicted effector binding element (EBE) site and 23 lost the EBE sequence altogether. Inoculation and bioinformatics studies suggest that the Tal7b target in one of the Tal7b-specific QTL, qBB-tal7b-8, is a disease susceptibility gene and that the resistance mechanism for this locus may be through loss of susceptibility. Our work demonstrates that minor-effect virulence factors significantly contribute to disease and provide a potential new approach to identify effective disease resistance.

Item Type:Article
Business groups:Horticulture and Forestry Science
Additional Information:Open access
Subjects:Science > Biology > Genetics > Quantitative genetics (esp. Quantitative Trait Loci (QTL) articles)
Plant culture > Field crops > Rice
Plant pests and diseases
Deposited On:05 Jul 2021 03:23
Last Modified:03 Sep 2021 16:46

Repository Staff Only: item control page

Downloads

Downloads per month over past year

View more statistics