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Serum proteomes of Santa Gertrudis cattle before and after infestation with Rhipicephalus australis ticks

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Raza, A., Schulz, B. L., Nouwens, A., Jackson, L. A., Piper, E. K., James, P., Jonsson, N. N. and Tabor, A. E. (2021) Serum proteomes of Santa Gertrudis cattle before and after infestation with Rhipicephalus australis ticks. Parasite Immunology, 43 (7). e12836. ISSN 0141-9838

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Article Link(s): https://doi.org/10.1111/pim.12836

Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/pim.12836

Abstract

Previous studies have applied genomics and transcriptomics to identify immune and genetic markers as key indicator traits for cattle tick susceptibility/resistance; however, results differed between breeds, and there is lack of information on the use of host proteomics. Serum samples from Santa Gertrudis cattle (naïve and phenotyped over 105 days as tick-resistant [TR] or tick-susceptible [TS]) were used to conduct differential abundance analyses of protein profiles. Serum proteins were digested into peptides followed by identification and quantification using sequential window acquisition of all instances of theoretical fragment ion mass spectrometry. Before tick infestation, abundance of 28 proteins differed significantly (adjusted P < 10−5) between TR and TS. These differences were also observed following tick infestation (TR vs TS) with a further eight differentially abundant proteins in TR cattle, suggesting possible roles in adaptive responses. The intragroup comparisons (TS-0 vs TS and TR-0 vs TR) showed that tick infestation elicited quite similar responses in both groups of cattle, but with relatively stronger responses in TR cattle. Many of the significantly differentially abundant proteins in TR Santa Gertrudis cattle (before and after tick infestation) were associated with immune responses including complement factors, chemotaxis for immune cells and acute-phase responses.

Item Type:Article
Business groups:Biosecurity Queensland
Keywords:biomarker discovery cattle host resistance proteomics Rhipicephalus australis Santa Gertrudis
Subjects:Science > Entomology
Animal culture > Cattle
Veterinary medicine > Veterinary parasitology
Deposited On:31 May 2021 07:00
Last Modified:07 Jun 2021 06:30

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