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Detection and Distribution of Viruses Infecting Garlic Crops in Australia

Cremer, J., Campbell, P., Steele, V., Persley, D., Thomas, J., Harper, S. and Gambley, C. (2021) Detection and Distribution of Viruses Infecting Garlic Crops in Australia. Plants, 10 (5). ISSN 2223-7747

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Article Link(s): https://doi.org/10.3390/plants10051013

Abstract

The distribution of viruses in eastern Australian field garlic was evaluated. Detection assays were developed that involved generic RT-PCR for viruses in the Allexivirus, Carlavirus and Potyvirus genera followed by virus-specific colorimetric dot-blot hybridization. Assays targeted the potyviruses (onion yellow dwarf virus (OYDV), shallot yellow stripe virus (SYSV), and leek yellow stripe virus (LYSV)), the carlaviruses (garlic common latent virus (GCLV) and shallot latent virus (SLV)), and the allexiviruses (garlic viruses A, B, C, X (GarVA, -B, -C, -X) and shallot virus X (ShVX)). Virus incidence in crops was consistently high, with most plants infected with at least one virus from each genus. OYDV, LYSV, SLV, and GCLV were commonly detected. Three of the four allexiviruses were in all districts surveyed but varied in incidence, whereas ShVX and SYSV were not detected. There was no association between virus species complement and bulb size, indicating size is not a good predictor of the virus status of planting material. The variation of virus incidence across different Australian growing districts and in different cultivars implies multiple introductions of viruses rather than spread within the country. The genetic diversity observed within coat protein sequences of some virus species also supports multiple separate introductions.

Item Type:Article
Business groups:Horticulture and Forestry Science
Additional Information:Open access
Keywords:garlic viruses large-scale survey molecular indexing genetic diversity
Subjects:Agriculture > Agriculture (General) > Methods and systems of culture. Cropping systems
Plant culture > Food crops
Plant pests and diseases
Plant pests and diseases > Plant pathology
Deposited On:31 May 2021 00:13
Last Modified:03 Sep 2021 16:46

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