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Dual RNA-Sequencing to Elucidate the Plant-Pathogen Duel

Naidoo, S., Visser, E. A., Zwart, L., Toit, Y. D., Bhadauria, V. and Shuey, L. S. (2018) Dual RNA-Sequencing to Elucidate the Plant-Pathogen Duel. Current issues in molecular biology, 27 . pp. 127-142. ISSN 14673045 (ISSN)

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Article Link(s): https://doi.org/10.21775/cimb.027.127

Abstract

RNA-sequencing technology has been widely adopted to investigate host responses during infection with pathogens. Dual RNA-sequencing (RNA-seq) allows the simultaneous capture of pathogen specific transcripts during infection, providing a more complete view of the interaction. In this review, we focus on the design of dual RNA-seq experiments and the application of downstream data analysis to gain biological insight into both sides of the interaction. Recent literature in this area demonstrates the power of the dual RNA-seq approach and shows that it is not limited to model systems where genomic resources are available. A reduction in sequencing cost and single cell transcriptomics coupled with protein and metabolite level dual approaches are set to enhance our understanding of plant-pathogen interactions. Sequencing costs continue to decrease and single cell transcriptomics is becoming more feasible. In combination with proteomics and metabolomics studies, these technological advances are likely to contribute to our understanding of the temporal and spatial aspects of dynamic plant-pathogen interactions.

Item Type:Article
Business groups:Horticulture and Forestry Science
Additional Information:Open access
Keywords:transcriptome chromosomal mapping disease resistance fungus gene library genetic marker genetics high throughput sequencing host pathogen interaction immunology metabolism microbiology pathogenicity plant plant disease plant genome procedures Chromosome Mapping Fungi Genetic Markers Genome, Plant High-Throughput Nucleotide Sequencing Host-Pathogen Interactions Plant Diseases Plants
Subjects:Science > Botany > Genetics
Plant pests and diseases
Plant pests and diseases > Plant pathology
Deposited On:09 Mar 2021 04:36
Last Modified:03 Sep 2021 16:46

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