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Gene introgression in assessing fitness costs associated with phosphine resistance in the rusty grain beetle

Singarayan, V. T., Jagadeesan, R., Nayak, M. K., Ebert, P. R. and Daglish, G. J. (2021) Gene introgression in assessing fitness costs associated with phosphine resistance in the rusty grain beetle. Journal of Pest Science . ISSN 1612-4766

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Article Link(s): https://doi.org/10.1007/s10340-020-01315-6

Abstract

The current study investigates the fitness cost associated with phosphine resistance in the rusty grain beetle, Cryptolestes ferrugineus (Stephens), a problematic pest in the stored commodities that has developed strong resistance to fumigant phosphine. Three characterised insect strains: the susceptible (Ref-S), the strongly resistant (Ref-R), the introgressed resistant (Intro-R) and a segregating population (F25) derived from crossing the Ref-S and Ref-R strains were used in this study. Intro-R was developed by introgressing two phosphine resistance genes, cf_rph1 and cf_rph2 into Ref-S, aimed to reduce the influence of background genetic factors. Intro-R exhibited 592 × resistance to phosphine and homozygous for strong resistance allele, cf_rph2 (L73N). Two key fitness cost criteria, developmental time and fecundity, were assessed under optimal and suboptimal conditions (less favourable diet and low temperature). There was no significant difference in developmental time and fecundity between Ref-S and either Intro-R strain or F25 under optimal conditions. When challenged with a less favourable diet, cracked wheat + cracked sorghum (CW + CS), or exposed to a low temperature (22 °C), both Intro-R and Ref-S had similar developmental time and total numbers of F1 progeny, confirming the absence of significant fitness effects expressed in these conditions. Therefore, we conclude that strongly phosphine resistant C. ferrugineus are unlikely to incur potential fitness costs. This finding will have implications towards developing strategies to manage this pest.

Item Type:Article
Business groups:Crop and Food Science
Additional Information:#
Keywords:Biological cost Developmental time Reproduction Suboptimal condition Low temperature
Subjects:Science > Botany > Genetics
Plant culture > Field crops > Grain. Cereals
Plant pests and diseases > Pest control and treatment of diseases. Plant protection
Deposited On:11 Jan 2021 23:21
Last Modified:03 Sep 2021 16:46

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