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Multivariate ratio analysis and DNA markers reveal a new Australian species and three synonymies in eucalypt-gall-associated Megastigmus (Hymenoptera: Megastigmidae)

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Le, N. H., Nahrung, H. F., Morgan, J. A. T. and Lawson, S. A. (2020) Multivariate ratio analysis and DNA markers reveal a new Australian species and three synonymies in eucalypt-gall-associated Megastigmus (Hymenoptera: Megastigmidae). Bulletin of Entomological Research . pp. 1-16. ISSN 0007-4853

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Article Link(s): https://doi.org/10.1017/S000748532000022X

Publisher URL: https://www.cambridge.org/core/article/multivariate-ratio-analysis-and-dna-markers-reveal-a-new-australian-species-and-three-synonymies-in-eucalyptgallassociated-megastigmus-hymenoptera-megastigmidae/34DB2DFD45F35937D33E67FAB74AC42C

Abstract

The genus Megastigmus Dalman, 1820 (Hymenoptera: Megastigmidae) contains potential biocontrol agents of the invasive eucalypt galling chalcid Leptocybe spp. (Hymenoptera: Eulophidae), with several species reported in various parts of the world. Species discrimination is challenging due to intraspecific morphological variation, difficulty in measuring sizes of body parts, and the lack of information regarding the global distribution of parasitic Megastigmus. We used two species commonly associated with Leptocybe in its native range to review taxonomic methods and determine the most reliable morphological characters in species delimitation. We examined size variation of body characters, and conducted species discrimination using multivariate ratio analysis, mitochondrial Cytochrome c oxidase subunit 1 (COI) and nuclear 28S rDNA (28S) sequences. Morphological traits were effective in species delimitation yet revealed high variation in several characters employed in current keys. Knowledge generated on morphology and DNA justified the description of a new species, M. manonae, sp. n., the first record of M. pretorianensis in Australia, and revised diagnostic characters for M. zvimendeli. Based on these diagnostic characters and molecular data, we synonymize three species (M. judikingae, syn. n., from Australia, M. sichuanensis, syn. n., from China and M. icipeensis, syn. n., from Kenya) with M. zvimendeli. Our findings highlight the importance of molecular markers in assisting taxonomic decision-making and the need for coordinated work in identifying Megastigmus associated with Leptocybe spp.

Item Type:Article
Business groups:Animal Science
Keywords:Leptocybe Megastigmus manonae Megastigmus zvimendeli morphology parasitoids species discrimination
Subjects:Science > Entomology
Science > Biology > Genetics
Plant pests and diseases > Pest control and treatment of diseases. Plant protection
Forestry > Research. Experimentation
Deposited On:02 Nov 2020 02:40
Last Modified:02 Nov 2020 04:29

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