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Identification of putative viroplasms within banana cells infected by banana streak MY virus

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Ngo, B. T. H., Webb, R., Crew, K. S., Vance, M. E., Thomas, J. E. and Geering, A. D. W. (2020) Identification of putative viroplasms within banana cells infected by banana streak MY virus. Journal of General Virology .

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Article Link(s): https://doi.org/10.1099/jgv.0.001498

Publisher URL: https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001498

Abstract

The badnavirus replication cycle is poorly understood and most knowledge is based on extrapolations from model viruses such as Cauliflower mosaic virus (CaMV). However, in contrast to CaMV, badnaviruses are thought not to produce viroplasms and therefore it has been a mystery as to where virion assembly occurs. In this study, ultrathin sections of a banana leaf infected with a badnavirus, banana streak MY virus (BSMYV), were examined by transmission electron microscopy. Electron-dense inclusion bodies (EDIBs) were sporadically distributed in parenchymatous tissues of the leaf, most commonly in the palisade and spongy mesophyll cells. These EDIBs had a characteristic structure, comprising an electron-dense core, a single, encircling lacuna and an outer ring of electron-dense material. However, much less frequently, EDIBs with two or three lacunae were observed. In the outer ring, densely packed virions were visible with a shape and size consistent with that expected for badnaviruses. Immunogold labelling was done with primary antibodies that detected the N-terminus of the capsid protein and strong labelling of the outer ring but not the central core or lacuna was observed. It is concluded that the EDIBs that were observed are equivalent in function to the viroplasms of CaMV, although obviously different in composition as there is not a paralogue of the transactivation/viroplasm protein in the badnavirus genome. It is postulated that production of a viroplasm could be a conserved characteristic of all members of the Caulimoviridae.

Item Type:Article
Business groups:Horticulture and Forestry Science
Keywords:banana streak disease virus morphogenesis badnavirus cytopathology replication cycle
Subjects:Plant culture > Food crops
Plant culture > Vegetables
Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Bananas
Plant pests and diseases
Plant pests and diseases > Plant pathology
Deposited On:06 Oct 2020 03:46
Last Modified:06 Oct 2020 03:46

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