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Genomic Regions Associated with Virulence in Pyrenophora teres f. teres Identified by Genome-Wide Association Analysis and Biparental Mapping

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Martin, A., Moolhuijzen, P., Tao, Y., McIlroy, J., Ellwood, S. R., Fowler, R. A., Platz, G. J., Kilian, A. and Snyman, L. (2020) Genomic Regions Associated with Virulence in Pyrenophora teres f. teres Identified by Genome-Wide Association Analysis and Biparental Mapping. Phytopathology, 110 (4). pp. 881-891.

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Article Link(s): https://doi.org/10.1094/PHYTO-10-19-0372-R

Publisher URL: https://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-10-19-0372-R

Abstract

Net form net blotch (NFNB), caused by the fungal pathogen Pyrenophora teres f. teres, is an important foliar disease present in all barley-producing regions of the world. This fungus is a hemibiotrophic and heterothallic ascomycete, where sexual recombination can lead to changes in disease expression in the host. Knowledge of the genetic architecture and genes involved in virulence is vital to increase the durability of NFNB resistance in barley cultivars. We used a genome-wide association mapping approach to characterize P. teres f. teres genomic regions associated with virulence in Australian barley cultivars. One hundred eighty-eight P. teres f. teres isolates collected across five Australian states were genotyped using Diversity Arrays Technology sequence markers and phenotyped across 20 different barley genotypes. Association mapping identified 14 different genomic regions associated with virulence, with the majority located on P. teres f. teres chromosomes 3 and 5 and one each present on chromosomes 1, 6, and 9. Four of the regions identified were confirmed by quantitative trait loci (QTL) mapping. The QTL regions are discussed in the context of their genomic architecture together with examination of their gene contents, which identified 20 predicted effectors. The number of QTL shown in this study at the population level clearly illustrates a complex genetic basis of P. teres f. teres virulence compared with pure necrotrophs, such as the wheat pathogens Parastagonospora nodorum and Parastagonospora tritici-repentis.

Item Type:Article
Business groups:Crop and Food Science
Keywords:candidate genes,disease control and pest management,genetics and resistance,genome-wide association mapping,Pyrenophora teres f. teres,QTL analysis,virulence
Subjects:Science > Invasive Species > Plants
Science > Botany > Genetics
Plant culture > Field crops > Barley
Plant pests and diseases
Plant pests and diseases > Plant pathology
Deposited On:31 Aug 2020 02:50
Last Modified:31 Aug 2020 02:50

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