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Bacteriophages to Control Campylobacter in Commercially Farmed Broiler Chickens, in Australia

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Chinivasagam, H.N., Estella, W., Maddock, L., Mayer, D.G., Weyand, C., Connerton, P. L. and Connerton, I. F. (2020) Bacteriophages to Control Campylobacter in Commercially Farmed Broiler Chickens, in Australia. Frontiers in Microbiology, 11 (632). ISSN 1664-302X

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Article Link(s): https://doi.org/10.3389/fmicb.2020.00632

Publisher URL: https://www.frontiersin.org/article/10.3389/fmicb.2020.00632

Abstract

This study describes the development and use of bacteriophage cocktails to control Campylobacter in broiler chickens, in a commercial setting, in Queensland Australia, following the birds from farm to the processing plant. The components of the bacteriophage cocktails were selected to be effective against the maximum number of Campylobacter jejuni and Campylobacter coli isolates encountered on SE Queensland farms. Farms were identified that had suitable Campylobacter target populations and phage were undetectable 1 week prior to the intended treatment. Cocktails of phages were administered at 47 days of age. Groups of study birds were slaughtered the following day, on-farm, at the end of flock transport to the plant, and at processing (approximately 28 h post-treatment). On Farm A, the phage treatment significantly reduced Campylobacter levels in the ceca at the farm in the range of 1–3 log<sub>10</sub> CFU/g (p = 0.007), compared to mock treated controls. However, individual birds sampled on farm (1/10) or following transport (2/10) exhibited high cecal Campylobacter counts with low phage titers, suggesting that treatment periods > 24 h may be required to ensure phage replication for effective biocontrol in vivo. At the time of the trial the control birds in Farm B were phage positive despite having been negative one week earlier. There was no significant difference in the cecal Campylobacter counts between the treatment and control groups following treatment but a fall of 1.7 log<sub>10</sub> CFU/g was observed from that determined from birds collected the previous week (p = 0.0004). Campylobacter isolates from both farms retained sensitivity to the treatment phages. These trials demonstrated bacteriophages sourced from Queensland farms have the potential to reduce intestinal Campylobacter levels in market ready broiler chickens.

Item Type:Article
Business groups:Animal Science
Additional Information:Open access
Keywords:Bacteriophage,Campylobacter,broiler chicken,Queensland (Australia),Poultry
Subjects:Animal culture > Poultry
Animal culture > Poultry > Chickens
Veterinary medicine > Veterinary microbiology
Veterinary medicine > Veterinary bacteriology
Deposited On:07 May 2020 03:08
Last Modified:07 May 2020 03:08

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