Login | Request Account (DAF staff only)

DArTseq Molecular Markers Associated with the Spiny-Tip Leaf Margin in Pineapple (Ananas comosus L.)

Sanewski, G. M. (2020) DArTseq Molecular Markers Associated with the Spiny-Tip Leaf Margin in Pineapple (Ananas comosus L.). Tropical Plant Biology, 13 . pp. 91-116. ISSN 1935-9764

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link(s): https://doi.org/10.1007/s12042-019-09245-z

Publisher URL: http://link.springer.com/article/10.1007/s12042-019-09245-z

Abstract

A genome-wide association study (GWAS) and quantitative trait loci (QTL) analysis using two bi-parental (parental cultivars Smooth Cayenne and MD-2) pineapple seedling populations segregating for spiny and spiny-tip leaf margin and 12 wild and pre-Columbian domesticated genotypes were used to identify single nucleotide polymorphism (SNP) and silicoDArT markers associated with the spiny-tip leaf margin phenotype in pineapple. One QTL between the nucleotide positions 14,355,639 and 14,368,806 on linkage group six (LG06) was identified using SNP markers and one QTL between the nucleotide positions 14,330,844 and 14,346,378 using silicoDArT markers. GWAS and QTL analysis methods identified the same most significantly associated SNP and silicoDArT markers. The most significantly associated SNP and silicoDArT markers were positioned at 14,355,639 and 14,341,745 bp respectively, on or very near, a zeaxanthin epoxidase (ZEP) gene, a key gene in the abscisic acid (ABA) pathway. Other associated genes with a high significance by GWAS analysis using at least two algorithms include a detoxification 33-like (DTX) and a resistance gene analog (RGA2-like). It is proposed that a polymorphism in the putative ZEP gene is the main causal variant associated with the spiny-tip leaf margin in ‘Smooth Cayenne’ pineapple and its descendants including ‘MD-2’.

Item Type:Article
Business groups:Horticulture and Forestry Science
Keywords:ABA Ananas Tetratricopeptide RGA DTX Pineapple SNPs Spines Zeaxanthin Epoxidase
Subjects:Science > Botany > Plant anatomy
Science > Botany > Genetics
Plant culture > Food crops
Plant culture > Fruit and fruit culture
Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Pineapple
Deposited On:29 Jan 2020 02:26
Last Modified:02 Jun 2020 00:59

Repository Staff Only: item control page