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An easy-to-perform, culture-free Campylobacter point-of-management assay for processing plant applications

Mason, M. G., Blackall, P. J., Botella, J. R. and Templeton, J. M. (2019) An easy-to-perform, culture-free Campylobacter point-of-management assay for processing plant applications. Journal of Applied Microbiology, Early (n/a). ISSN 1364-5072

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Article Link(s): https://doi.org/10.1111/jam.14509

Publisher URL: https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.14509

Abstract

Aims Current culture-based methods for detection and determination of Campylobacter levels on processed chickens takes at least two days. Here, we sought to develop a new complete, low-cost and rapid (approximately 2·5 hours) detection system requiring minimal operator input. Methods and Results We observed a strong correlation between culture-based cell counts and our ability to detect either Campylobacter jejuni or Campylobacter coli by Loop mediated isothermal amplification (LAMP) from the same samples. This knowledge was used to develop a rapid and simple five-step assay to quantify Campylobacter, which was subsequently assessed for its specificity, reproducibility and accuracy in quantifying Campylobacter levels from processed chickens. The assay was found to be highly specific for C. jejuni and C. coli and was capable of distinguishing between samples that are either within or exceeding the industry set target of 6,000 Campylobacter colony forming units (cfu) per carcass (equivalent to 12 cfu ml-1 of chicken rinse) with> 90% accuracy relative to culture-based methods. Conclusions Our method can reliably quantify Campylobacter counts of processed chickens with an accuracy comparable to culture-based assays but provides results within hours as opposed to days. Significance and Impact of the Study The research presented here will help improve food safety by providing fast Campylobacter detection that will enable the implementation of real-time risk management strategies in poultry processing plants to rapidly test processed chickens and identify effective intervention strategies. This technology is a powerful tool that can be easily adapted for other organisms and thus could be highly beneficial for a broad range of industries.

Item Type:Article
Business groups:Animal Science
Additional Information:Open access
Subjects:Agriculture > Agriculture (General) > Farm machinery and farm engineering
Animal culture > Poultry
Veterinary medicine > Veterinary microbiology
Deposited On:14 Jan 2020 02:38
Last Modified:14 Jan 2020 02:38

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