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Towards sub-species resolution profiling of plant and soil microbiomes

Dennis, P. G., Birt, H., Liew, E., Summerell, B., McTaggart, A. R., Shivas, R. and Pattison, T. (2019) Towards sub-species resolution profiling of plant and soil microbiomes. In: Australasian Plant Pathology Society Conference APPS 2019 Strong Foundations, Future Innovations, 25-28 November 2019, Melbourne, Australia.

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Abstract

Sequence-based studies to characterise microbial communities remain dominated by platforms that offer ultra-high output of short (100-250 bp), high-quality reads. This is largely because platforms capable of generating much longer, and potentially more informative reads (>10 kb), are prone to high error rates. Recent advances in library preparation and bioinformatics, both internationally and in our lab, make it possible to generate long-high-quality reads that can be used to profile microbial diversity at sub-species-resolution, and enhance the recovery of genomes from metagenomic datasets. Here, we present results from our work with the microbiomes of banana plants and soils and their interactions with Fusarium spp. This novel technology has the potential to revolutionise pathogen diagnostics and our understanding of pathogen-microbiome ecology.

Item Type:Conference or Workshop Item (Paper)
Business groups:Biosecurity Queensland
Subjects:Science > Botany > Genetics
Plant culture > Field crops
Plant culture > Horticulture. Horticultural crops
Plant pests and diseases
Plant pests and diseases > Plant pathology
Plant pests and diseases > Pest control and treatment of diseases. Plant protection
Deposited On:10 Dec 2019 03:40
Last Modified:10 Dec 2019 03:40

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