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The intrinsic and regulated proteomes of barley seeds in response to fungal infection

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Kerr, E. D., Phung, T. K., Caboche, C. H., Fox, G. P., Platz, G. J. and Schulz, B. L. (2019) The intrinsic and regulated proteomes of barley seeds in response to fungal infection. Analytical Biochemistry, 580 . pp. 30-35. ISSN 0003-2697

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Article Link: https://doi.org/10.1016/j.ab.2019.06.004

Publisher URL: http://www.sciencedirect.com/science/article/pii/S0003269719304038


Barley is an important cereal grain used for beer brewing, animal feed, and human food consumption. Fungal disease can impact barley production, as it causes substantial yield loss and lowers seed quality. We used sequential window acquisition of all theoretical ions mass spectrometry (SWATH-MS) to measure and quantify the relative abundance of proteins within seeds of different barley varieties under various fungal pathogen burdens (ProteomeXchange Datasets PXD011303 and PXD014093). Fungal burden in the leaves and stems of barley resulted in changes to the seed proteome. However, these changes were minimal and showed substantial variation among barley samples infected with different pathogens. The limited effect of intrinsic disease resistance on the seed proteome is consistent with the main mediators of disease resistance being present in the leaves and stems of the plant. The seeds of barley varieties accredited for use as malt had higher levels of proteins associated with starch synthesis and beer quality. The proteomic workflows developed and implemented here have potential application in quality control, breeding and processing of barley, and other agricultural products.

Item Type:Article
Subjects:Science > Botany > Genetics
Plant culture > Field crops > Barley
Plant pests and diseases
Live Archive:02 Jul 2019 00:15
Last Modified:03 Sep 2021 16:45

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