Login | Create Account (DAF staff only)

Global distribution of white spot syndrome virus genotypes determined using a novel genotyping assay

Oakey, J., Smith, C., Underwood, D., Afsharnasab, M., Alday-Sanz, V., Dhar, A., Sivakumar, S., Sahul Hameed, A. S., Beattie, K. and Crook, A. (2019) Global distribution of white spot syndrome virus genotypes determined using a novel genotyping assay. Archives of Virology . ISSN 1432-8798

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link(s): https://doi.org/10.1007/s00705-019-04265-2

Abstract

White spot disease, caused by infection with white spot syndrome virus (WSSV), is a serious panzootic affecting prawn aquaculture. The disease has spread rapidly around the prawn-culturing regions of the world through a number of previously identified mechanisms. The ability to distinguish and trace strains of WSSV is of great benefit to identify, and then limit, the translocation routes of the disease. Here, we describe a novel genotyping method using 34 short tandem repeat regions of the viral genome concurrently. This technique is highly sensitive to strain differences when compared to previous methods. The efficacy of the described method is demonstrated by testing WSSV isolates from around the globe, showing regional genotypic differences. The differences in the genotypes were used to create a global minimum spanning network, and in most cases the observed relationships were substantiated with verification of transboundary movement. This novel panel of STR markers will provide a valuable epidemiological tool for white spot disease. We have applied this to an outbreak of the disease in Queensland, Australia, that occurred in 2016. While the results indicate that the source of this outbreak currently remains cryptic, the analyses have provided valuable insights with which to further study the origins of the strains involved.

Item Type:Article
Business groups:Biosecurity Queensland
Subjects:Science > Biology > Genetics
Veterinary medicine > Veterinary virology
Veterinary medicine > Communicable diseases of animals (General)
Aquaculture and Fisheries > Fisheries > Shellfish fisheries
Deposited On:30 May 2019 02:28
Last Modified:30 May 2019 02:28

Repository Staff Only: item control page