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Potential for using pheromone trapping and molecular screening in phosphine resistance research

Daglish, G. J., Rajeswaran, J., Singarayan, V., Nath, N. S., Schlipalius, D. I., Ebert, P. R. and Nayak, M. K. (2018) Potential for using pheromone trapping and molecular screening in phosphine resistance research. In: Proceedings of the 12th International Working Conference on Stored Product Protection (IWCSPP), 7-11 October, 2018, Berlin, Germany..

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Article Link(s): https://ojs.openagrar.de/index.php/JKA/article/vie...

Abstract

Phosphine resistance monitoring typically involves bioassays of beetles from population samples collected from grain storage facilities. Insects are classified into susceptible or resistant phenotypes based on mortality or survival at one or more discriminating doses. Although valuable, phenotype testing has several drawbacks. First, phenotype testing needs live insects, and considerable effort is required to collect and maintain them before testing. Second, population samples may contain multiple genotypes expressing different levels of resistance that may not be distinguishable using discriminating dose bioassays. Third, collections are likely to be focussed around grain storages to maximise sampling success. Recent research shows that several key pests are actively dispersing through flight. The availability of commercial pheromone lures and recent advances in molecular screening provide an opportunity to provide information on resistance gene frequencies more broadly across the landscape. This approach is proving to be a valuable adjunct to traditional resistance testing in Australia.

Item Type:Conference or Workshop Item (Paper)
Business groups:Crop and Food Science
Subjects:Science > Entomology
Plant pests and diseases > Plant pathology
Plant pests and diseases > Pest control and treatment of diseases. Plant protection
Deposited On:17 Apr 2019 05:29
Last Modified:17 Apr 2019 05:29

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