Login | Create Account (DAF staff only)

Moult cycle specific differential gene expression profiling of the crab Portunus pelagicus

Kuballa, A. V., Holton, T. A., Paterson, B. and Elizur, A. (2011) Moult cycle specific differential gene expression profiling of the crab Portunus pelagicus. BMC Genomics, 12 , 147. pp. 1-18. ISSN 1471-2164

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link(s): http://doi.org/10.1186/1471-2164-12-147

Publisher URL: https://doi.org/10.1186/1471-2164-12-147

Abstract

Crustacean moulting is a complex process involving many regulatory pathways. A holistic approach to examine differential gene expression profiles of transcripts relevant to the moulting process, across all moult cycle stages, was used in this study. Custom cDNA microarrays were constructed for Portunus pelagicus. The printed arrays contained 5000 transcripts derived from both the whole organism, and from individual organs such as the brain, eyestalk, mandibular organ and Y-organ from all moult cycle stages.
A total of 556 clones were sequenced from the cDNA libraries used to construct the arrays. These cDNAs represented 175 singletons and 62 contigs, resulting in 237 unique putative genes. The gene sequences were classified into the following biological functions: cuticular proteins associated with arthropod exoskeletons, farnesoic acid O-methyltransferase (FaMeT), proteins belonging to the hemocyanin gene family, lectins, proteins relevant to lipid metabolism, mitochondrial proteins, muscle related proteins, phenoloxidase activators and ribosomal proteins. Moult cycle-related differential expression patterns were observed for many transcripts. Of particular interest were those relating to the formation and hardening of the exoskeleton, and genes associated with cell respiration and energy metabolism.
The expression data presented here provide a chronological depiction of the molecular events associated with the biological changes that occur during the crustacean moult cycle. Tracing the temporal expression patterns of a large variety of transcripts involved in the moult cycle of P. pelagicus can provide a greater understanding of gene function, interaction, and regulation of both known and new genes with respect to the moulting process.

Item Type:Article
Keywords:Arginine Kinase Moult Cycle Moult Stage Cuticular Protein Cuticle Protein
Subjects:Aquaculture and Fisheries > Fisheries > Fishery research
Deposited On:28 Mar 2019 01:05
Last Modified:28 Mar 2019 01:05

Repository Staff Only: item control page