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Deep sequencing-based transcriptome analysis of Plutella xylostella larvae parasitized by Diadegma semiclausum

Etebari, K., Palfreyman, R. W., Schlipalius, D., Nielsen, L. K., Glatz, R. V. and Asgari, S. (2011) Deep sequencing-based transcriptome analysis of Plutella xylostella larvae parasitized by Diadegma semiclausum. BMC Genomics, 12 , 446. ISSN 14712164 (ISSN)

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Article Link(s): http://doi.org/10.1186/1471-2164-12-446

Publisher URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-80052602353&partnerID=40&md5=7d65fc4e0a3cd068e7806b2f6d6c9636

Abstract

Background: Parasitoid insects manipulate their hosts' physiology by injecting various factors into their host upon parasitization. Transcriptomic approaches provide a powerful approach to study insect host-parasitoid interactions at the molecular level. In order to investigate the effects of parasitization by an ichneumonid wasp (Diadegma semiclausum) on the host (Plutella xylostella), the larval transcriptome profile was analyzed using a short-read deep sequencing method (Illumina). Symbiotic polydnaviruses (PDVs) associated with ichneumonid parasitoids, known as ichnoviruses, play significant roles in host immune suppression and developmental regulation. In the current study, D. semiclausum ichnovirus (DsIV) genes expressed in P. xylostella were identified and their sequences compared with other reported PDVs. Five of these genes encode proteins of unknown identity, that have not previously been reported.Results: De novo assembly of cDNA sequence data generated 172,660 contigs between 100 and 10000 bp in length; with 35% of > 200 bp in length. Parasitization had significant impacts on expression levels of 928 identified insect host transcripts. Gene ontology data illustrated that the majority of the differentially expressed genes are involved in binding, catalytic activity, and metabolic and cellular processes. In addition, the results show that transcription levels of antimicrobial peptides, such as gloverin, cecropin E and lysozyme, were up-regulated after parasitism. Expression of ichnovirus genes were detected in parasitized larvae with 19 unique sequences identified from five PDV gene families including vankyrin, viral innexin, repeat elements, a cysteine-rich motif, and polar residue rich protein. Vankyrin 1 and repeat element 1 genes showed the highest transcription levels among the DsIV genes.Conclusion: This study provides detailed information on differential expression of P. xylostella larval genes following parasitization, DsIV genes expressed in the host and also improves our current understanding of this host-parasitoid interaction. © 2011 Etebari et al; licensee BioMed Central Ltd.

Item Type:Article
Keywords:arginase catalase cecropin cecropin E ceramidase complementary DNA cysteine proteinase fascin flap endonuclease gloverin heat shock protein 90 insulin receptor lipocalin lysozyme moricin multidrug resistance protein 2 nicotinamide adenine dinucleotide phosphate polypeptide antibiotic agent proline rich protein RecQ helicase repeat element 1 RNA directed DNA polymerase serine proteinase syntaxin transcriptome transferrin triacylglycerol lipase unclassified drug vankyrin viral innexin article catalysis consensus sequence controlled study Diadegma semiclausum down regulation gene expression gene identification gene locus gene overexpression gene sequence genetic transcription host parasite interaction insect genetics larva Lepidoptera nonhuman nucleotide sequence parasitism Plutella xylostella Polydnaviridae protein assembly protein binding protein motif quantitative analysis reverse transcription polymerase chain reaction unindexed sequence upregulation virus like agent wasp animal DNA sequence gene gene expression profiling genetics high throughput sequencing methodology moth parasitology physiology virology Hexapoda Ichneumonidae Ichnovirus Animals Genes, Insect High-Throughput Nucleotide Sequencing Host-Parasite Interactions Moths Sequence Analysis, DNA Wasps
Subjects:Science > Entomology
Deposited On:27 Mar 2019 03:56
Last Modified:27 Mar 2019 03:56

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