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Capsular serotyping of Pasteurella multocida from various animal hosts - A comparison of phenotypic and genotypic methods

Arumugam, N. D., Ajam, N., Blackall, P. J., Asiah, N. M., Ramlan, M., Maria, J., Yuslan, S. and Thong, K. L. (2011) Capsular serotyping of Pasteurella multocida from various animal hosts - A comparison of phenotypic and genotypic methods. Tropical Biomedicine, 28 (1). pp. 55-63. ISSN 01275720 (ISSN)

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Article Link(s): http://www.msptm.org/files/55_-_63_Arumugam_ND.pdf

Abstract

One hundred and fourteen strains of Pasteurella multocida were isolated from different domestic animals species (cattle, buffalo, sheep, goat, pig, rabbit, dog, cat), avian species (chicken, duck, turkey) and wild animals (deer, tiger, orang utan, marmoset). The serogroups of P. multocida were determined by both conventional capsular serotyping and a multiplex PCR assay targeting specific capsular genes. Based on the conventional serotyping method, the 114 strains of P. multocida were subtyped into 55 species-specific (untypeable strains) P. multocida, 15 serogroup A, 23 serogroup B and 21 serogroup D. Based on the multiplex PCR assay on the specific capsular genes associated with each serogroup, the 114 strains were further divided to 22 species-specific P. multocida (KMT1 - 460 bp), 53 serogroup A (A - 1,044 bp), 33 serogroup B (B - 760 bp) and 6 serogroup D (D - 657 bp). No serogroup E (511 bp) or F (851 bp) was detected among the Malaysian P. multocida. PCR-based typing was more discriminative and could further subtype the previously untypeable strains. Overall, there was a significant and positive correlation between both methods in serogrouping P. multocida (r = 0.7935; p < 0.4893). Various serogroups of P. multocida were present among the livestock with 75% of the strains belonging to serogroups A or B. PCR serotyping was therefore a highly species-specific, sensitive and robust method for detection and differentiation of P. multocida serogroups compared to conventional serotyping. To the best of our knowledge, this is the first report from Malaysia of the application of a PCR to rapidly define the species-specific P. multocida and its serogroups as an important zoonotic pathogen in Malaysia.

Item Type:Article
Keywords:bacterial DNA animal animal disease article bacterial membrane classification domestic animal genetics genotype immunology isolation and purification Malaysia microbiology molecular typing Pasteurella multocida pasteurellosis phenotype physiology polymerase chain reaction sensitivity and specificity serotyping wild animal Animals Animals, Domestic Animals, Wild Bacterial Capsules DNA, Bacterial Pasteurella Infections
Subjects:Veterinary medicine > Veterinary bacteriology
Deposited On:22 Mar 2019 01:44
Last Modified:22 Mar 2019 01:44

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