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Potential Use of Genetically Engineered Variable Lymphocyte Receptor B Specific to Avian Influenza Virus H9N2

Im, S. P., Kim, J., Lee, J. S., Kim, S. W., Jung, J. W., Lazarte, J. M. S., Kim, J. Y., Kim, Y. R., Lee, J. H., Chong, R. S. M. and Jung, T. S. (2018) Potential Use of Genetically Engineered Variable Lymphocyte Receptor B Specific to Avian Influenza Virus H9N2. Journal of immunology, 201 (10). pp. 3119-3128. ISSN 0022-1767

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Article Link(s): http://dx.doi.org/10.4049/jimmunol.1800981

Abstract

The variable lymphocyte receptor (VLR) B of jawless vertebrates functions as a secreted Ab of jawed vertebrates and has emerged as an alternative Ab with a single polypeptide chain. After observing an upregulated VLRB response in hagfish immunized with avian influenza virus (AIV) subtype H9N2, we screened AIV H9N2-specific VLRB using a mammalian expression system. To improve the binding avidity of the Ag-specific VLRB to the Ag, we enabled multimerization of the VLRB by conjugating it with C-terminal domain of human C4b-binding protein. To dramatically enhance the expression and secretion of the Ag-specific VLRB, we introduced a glycine-serine linker and the murine Ig kappa leader sequence. The practical use of the Ag-specific VLRB was also demonstrated through various immunoassays, detected by anti-VLRB Ab (11G5). Finally, we found that the Ag-specific VLRB decreased the infectivity of AIV H9N2. Together, our findings suggest that the generated Ag-specific VLRB could be used for various immunoapplications.

Item Type:Article
Business groups:Biosecurity Queensland
Subjects:Veterinary medicine > Veterinary virology
Veterinary medicine > Diseases of special classes of animals > Avian
Deposited On:05 Mar 2019 23:26
Last Modified:05 Mar 2019 23:26

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