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Presence of Nicotinamide Adenine Dinucleotide–Independent Haemophilus paragallinarum in Mexico

Garcia, A.F., Angulo, E., Blackall, P.J. and Ortiz, A.M. (2004) Presence of Nicotinamide Adenine Dinucleotide–Independent Haemophilus paragallinarum in Mexico. Avian Diseases, 48 (2). pp. 452-429.

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Two isolates of Haemophilus paragallinarum were obtained from a layer chicken in Mexico. The isolates were confirmed as H. paragallinarum by polymerase chain reaction and conventional biochemical identification. The isolates were nicotinamide adenine dinucleotide (NAD) independent—growing on blood agar without the need of a nurse colony as well as on a complex medium that lacked both NAD and chicken serum. Both isolates were pathogenic, causing the typical clinical signs of infectious coryza in susceptible chickens. One isolate was Page serovar B/Kume serovar B-1 and the other isolate was Page serovar C/Kume serovar C-2. The isolates were associated with a field outbreak that involved an egg drop of 20% over a 3 wk period and a doubling of weekly mortality (from 0.1% to 0.2%).
This is the first report of NAD-independent H. paragallinarum outside South Africa and is the first time that NADindependent H. paragallinarum of serovar B has been reported.

Abbreviations: NAD ¼ nicotinamide adenine dinucleotide; NAM ¼ nicotinamide; PCR ¼ polymerase chain reaction; TM ¼ complete growth medium without chicken serum or nicotinamide adenine dinucleotide; TM/SN ¼ complete growth medium that contains both chicken serum and nicotinamide adenine dinucleotide

Item Type:Article
Additional Information:© American Association of Avian Pathologists.
Keywords:H. paragallinarum; Nicotinamide adenine dinucleotide; chicken serum.
Subjects:Veterinary medicine > Diseases of special classes of animals > Avian
Live Archive:05 Jan 2006
Last Modified:03 Sep 2021 16:47

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