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Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population

Bossa-Castro, A. M., Tekete, C., Raghavan, C., Delorean, E. E., Dereeper, A., Dagno, K., Koita, O., Mosquera, G., Leung, H., Verdier, V. and Leach, J. E. (2018) Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population. Plant Biotechnology Journal, 16 (9). pp. 1559-1568. ISSN 1467-7652

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Article Link(s): http://dx.doi.org/10.1111/pbi.12895

Abstract

Quantitative trait loci (QTL) that confer broad-spectrum resistance (BSR), or resistance that is effective against multiple and diverse plant pathogens, have been elusive targets of crop breeding programs. Multi-parent Advanced Generation Inter-Cross (MAGIC) populations, with their diverse genetic composition and high levels of recombination, are potential resources for identification of QTL for BSR. In this study, a rice MAGIC population was used to map QTL conferring BSR to two major rice diseases, bacterial leaf streak (BLS) and bacterial blight (BB), caused by Xanthomonas oryzae pathovars (pv.) oryzicola (Xoc) and oryzae (Xoo), respectively. Controlling these diseases is particularly important in Sub-Saharan Africa, where no sources of BSR are currently available in deployed varieties. The MAGIC founders and lines were genotyped by sequencing and phenotyped in the greenhouse and field by inoculation with multiple strains of Xoc and Xoo. A combination of genome-wide association studies (GWAS) and interval mapping analyses revealed 11 BSR QTL, effective against both diseases, and three pathovar-specific QTL. The most promising BSR QTL (qXO-2-1, qXO-4-1 and qXO-11-2) conferred resistance to more than nine Xoc and Xoo strains. GWAS detected 369 significant SNP markers with distinguishable phenotypic effects, allowing the identification of alleles conferring disease resistance and susceptibility. The BSR and susceptibility QTL will improve our understanding of the mechanisms of both resistance and susceptibility in the long term, and will be immediately useful resources for rice breeding programs. This article is protected by copyright. All rights reserved.

Item Type:Article
Business groups:Horticulture and Forestry Science
Additional Information:Open access
Keywords:broad-spectrum resistance MAGIC Xanthomonas oryzae Africa bacterial leaf streak bacterial blight
Subjects:Science > Botany > Genetics
Plant culture > Seeds. Seed technology
Plant culture > Propagation
Plant culture > Food crops
Plant culture > Field crops > Rice
Plant pests and diseases > Individual or types of plants or trees > Rice
Deposited On:05 Dec 2018 01:30
Last Modified:05 Dec 2018 01:30

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