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Whole Genome Sequencing Reveals Potential New Targets for Improving Nitrogen Uptake and Utilization in Sorghum bicolor

Massel, Karen and Campbell, Bradley and Mace, Emma and Tai, Shuaishuai and Tao, Yongfu and Worland, Belinda and Jordan, David and Botella, Jimmy and Godwin, Ian (2016) Whole Genome Sequencing Reveals Potential New Targets for Improving Nitrogen Uptake and Utilization in Sorghum bicolor. Frontiers in Plant Science, 7 (1544). ISSN 1664-462X

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Article Link(s): http://dx.doi.org/10.3389/fpls.2016.01544

Publisher URL: http://journal.frontiersin.org/article/10.3389/fpls.2016.01544

Abstract

Nitrogen (N) fertilizers are a major agricultural input where more than 100 million tons are supplied annually. Cereals are particularly inefficient at soil N uptake, where the unrecovered nitrogen causes serious environmental damage. Sorghum bicolor (sorghum) is an important cereal crop, particularly in resource-poor semi-arid regions, and is known to have a high NUE in comparison to other major cereals under limited N conditions. This study provides the first assessment of genetic diversity and signatures of selection across 230 fully sequenced genes putatively involved in the uptake and mobilization of N from a diverse panel of sorghum lines. This comprehensive analysis reveals an overall reduction in diversity as a result of domestication and a total of 128 genes displaying signatures of purifying selection, thereby revealing possible gene targets to improve NUE in sorghum and cereals alike. A number of key genes appear to have been involved in selective sweeps, reducing their sequence diversity. The ammonium transporter (AMT) genes generally had low allelic diversity, whereas a substantial number of nitrate/peptide transporter 1 (NRT1/PTR) genes had higher nucleotide diversity in domesticated germplasm. Interestingly, members of the distinct race Guinea margaritiferum contained a number of unique alleles, and along with the wild sorghum species, represent a rich resource of new variation for plant improvement of NUE in sorghum.

Item Type:Article
Business groups:Crop and Food Science
Keywords:Nitrogen uptake and mobilization,Nitrogen use efficiency (NUE),Sorghum (sorghum bicolor),Domestication,selection
Subjects:Agriculture > Agriculture (General) > Soils. Soil science
Agriculture > Agriculture (General) > Fertilisers
Plant culture > Field crops > Sorghum
Deposited On:19 Jan 2017 01:53
Last Modified:19 Jan 2017 01:53

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