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Detection of infectious spleen and kidney necrosis virus (ISKNV) and turbot reddish body iridovirus (TRBIV) from archival ornamental fish samples

Go, J. and Waltzek, T. B. and Subramaniam, K. and Yun, S. C. and Groff, J. M. and Anderson, I. G. and Chong, R. and Shirley, I. and Schuh, J. C. L. and Handlinger, J. H. and Tweedie, A. and Whittington, R. J. (2016) Detection of infectious spleen and kidney necrosis virus (ISKNV) and turbot reddish body iridovirus (TRBIV) from archival ornamental fish samples. Diseases of Aquatic Organisms, 122 (2). pp. 105-123.

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Article Link(s): http://www.int-res.com/articles/dao2016/122/d122p1...

Publisher URL: http://www.int-res.com/abstracts/dao/v122/n2/p105-123

Abstract

Although infections caused by megalocytiviruses have been reported from a wide range of finfish species for several decades, molecular characterisation of the viruses involved has been undertaken only on more recent cases. Sequence analysis of the major capsid protein and adenosine triphosphatase genes is reported here from formalin-fixed, paraffin-embedded material from 2 archival ornamental fish cases from 1986 and 1988 in conjunction with data for a range of genes from fresh frozen tissues from 5 cases obtained from 1991 through to 2010. Turbot reddish body iridovirus (TRBIV) genotype megalocytiviruses, previously not documented in ornamental fish, were detected in samples from 1986, 1988 and 1991. In contrast, megalocytiviruses from 1996 onwards, including those characterised from 2002, 2006 and 2010 in this study, were almost indistinguishable from infectious spleen and kidney necrosis virus (ISKNV). Three of the species infected with TRBIV-like megalocytiviruses from 1986 to 1991, viz. dwarf gourami Trichogaster lalius (formerly Colisa lalia), freshwater angelfish Pterophyllum scalare and oscar Astronotus ocellatus, were infected with ISKNV genotype megalocytiviruses from 2002 to 2010. The detection of a TRBIV genotype isolate in ornamental fish from 1986 represents the index case, confirmed by molecular sequence data, for the genus Megalocytivirus.

Item Type:Article
Business groups:Biosecurity Queensland
Keywords:Megalocytivirus · Iridovirus · Phylogenetics · Molecular epidemiology · Ornamental fish · ISKNV · TRBIV · Red seabream iridovirus · RSIV · Taxonomy
Subjects:Veterinary medicine > Veterinary virology
Veterinary medicine > Veterinary epidemiology. Epizootiology
Aquaculture and Fisheries > Fisheries > Fishery research
Deposited On:18 Jan 2017 01:35
Last Modified:18 Jan 2017 01:35

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