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Cryopreservation of somatic embryos for avocado germplasm conservation

O’Brien, C. and Constantin, M. and Walia, A. and Yiing, J. Lim Yuan and Mitter, N. (2016) Cryopreservation of somatic embryos for avocado germplasm conservation. Scientia Horticulturae, 211 . pp. 328-335. ISSN 0304-4238

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Article Link(s): http://dx.doi.org/10.1016/j.scienta.2016.09.008

Publisher URL: http://www.sciencedirect.com/science/article/pii/S0304423816304381

Abstract

Presently avocado germplasm is conserved ex situ in the form of field repositories across the globe including Australia. The maintenance of germplasm in the field is costly, labour and land intensive, exposed to natural disasters and always at the risk of abiotic and biotic stresses. The aim of this study was to overcome these problems using cryopreservation to store avocado (Persea americana Mill.) somatic embryos (SE). Two vitrification-based methods of cryopreservation were optimised (cryovial and droplet-vitrification) using four avocado cultivars (‘A10′, ‘Reed’, ‘Velvick’ and ‘Duke-7′). SE of the four cultivars were stored for short-term (one hour) in liquid nitrogen using the cryovial-vitrification method and showed a viability of 91%, 73%, 86% and 80% respectively. While when using the droplet vitrification method viabilities of 100%, 85% and 93% were recorded for ‘A10′, ‘Reed’ and ‘Velvick’. For long-term storage, SE of cultivars ‘A10′, ‘Reed’ and ‘Velvick’ were successfully recovered with viability of 65–100% after 3 months of LN storage. For cultivar ‘Reed’ and ‘Velvick’ SE were recovered after 12 months of LN storage with viability of 67% and 59%, respectively. The outcome of this work contributes towards the establishment of a cryopreservation protocol that is applicable across multiple avocado cultivars.

Item Type:Article
Business groups:Horticulture and Forestry Science
Keywords:Avocado Cryopreservation Cryovial-vitrification Droplet-vitrification Somatic embryo
Subjects:Plant culture > Seeds. Seed technology
Plant culture > Fruit and fruit culture
Deposited On:25 Sep 2016 23:32
Last Modified:25 Sep 2016 23:32

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