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Genetic diversity of stay-green sorghums and their derivatives revealed by microsatellites

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Galyuon, I. K., Madhusudhana, R., Borrell, A. K., Hash, T. C. and Howarth, C. J. (2016) Genetic diversity of stay-green sorghums and their derivatives revealed by microsatellites. African Journal of Biotechnology, 15 (25). pp. 1363-1374. ISSN 1684-5315

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Article Link: http://www.academicjournals.org/journal/AJB/articl...

Abstract

The genetic variability of 28 sorghum genotypes of known senescence phenotype was investigated using 66 SSR markers well-distributed across the sorghum genome. The genotypes of a number of lines from breeding programmes for stay-green were also determined. This included lines selected phenotypically for stay-green and also RSG 03123, a marker-assisted backcross progeny of R16 (recurrent parent) and B35 (stay-green donor). A total of 419 alleles were detected with a mean of 6.2 per locus. The number of alleles ranged from one for Xtxp94 to 14 for Xtxp88. Chromosome SBI-10 had the highest mean number of alleles (8.33), while SBI-05 had the lowest (4.17). The PIC values obtained ranged from zero to 0.89 in Xtxp94 and Xtxp88, respectively, with a mean of 0.68. On a chromosome basis, mean PIC values were highest in SBI-10 (0.81) and lowest in SBI-05 (0.53). Most of the alleles from B35 in RSG 03123 were found on chromosomes SBI-01, SBI-02 and SBI-03, confirming the successful introgression of quantitative trait loci associated with stay-green from B35 into the senescent background R16. However, the alternative stay-green genetic sources were found to be distinct based on either all the SSRs employed or using only those associated with the stay-green trait in B35. Therefore, the physiological and biochemical basis of each stay-green source should be evaluated in order to enhance the understanding of the functioning of the trait in the various backgrounds. These genetic sources of stay-green could provide a valuable resource for improving this trait in sorghum breeding programmes.

Item Type:Article
Business groups:Crop and Food Science
Keywords:Simple sequence repeats, sorghum, stay-green, genetic diversity.
Subjects:Science > Botany > Genetics
Plant culture > Field crops > Grain. Cereals
Live Archive:17 Aug 2016 04:02
Last Modified:03 Sep 2021 16:50

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