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Immunogenicity of Outer Membrane Proteins VirB9-1 and VirB9-2, a Novel Nanovaccine against Anaplasma marginale

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Zhao, L., Mahony, D., Cavallaro, A. S., Zhang, B., Zhang, J., Deringer, J. R., Zhao, C.-X., Brown, W. C., Yu, C., Mitter, N. and Middelberg, A. P. J. (2016) Immunogenicity of Outer Membrane Proteins VirB9-1 and VirB9-2, a Novel Nanovaccine against Anaplasma marginale. PLOS ONE, 11 (4). e0154295. ISSN 1932-6203

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Article Link: http://dx.doi.org/10.1371/journal.pone.0154295


Anaplasma marginale is the most prevalent tick-borne livestock pathogen and poses a significant threat to cattle industry. In contrast to currently available live blood-derived vaccines against A. marginale, alternative safer and better-defined subunit vaccines will be of great significance. Two proteins (VirB9-1 and VirB9-2) from the Type IV secretion system of A. marginale have been shown to induce humoral and cellular immunity. In this study, Escherichia coli were used to express VirB9-1 and VirB9-2 proteins. Silica vesicles having a thin wall of 6 nm and pore size of 5.8 nm were used as the carrier and adjuvant to deliver these two antigens both as individual or mixed nano-formulations. High loading capacity was achieved for both proteins, and the mouse immunisation trial with individual as well as mixed nano-formulations showed high levels of antibody titres over 107 and strong T-cell responses. The mixed nano-formulation also stimulated high-level recall responses in bovine T-cell proliferation assays. These results open a promising path towards the development of efficient A. marginale vaccines and provide better understanding on the role of silica vesicles to deliver multivalent vaccines as mixed nano-formulations able to activate both B-cell and T-cell immunity, for improved animal health.

Item Type:Article
Business groups:Animal Science
Subjects:Animal culture > Cattle
Veterinary medicine > Communicable diseases of animals (General)
Veterinary medicine > Veterinary parasitology
Live Archive:04 Aug 2016 05:53
Last Modified:03 Sep 2021 16:50

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