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New yellow head virus genotype (YHV7) in giant tiger shrimp Penaeus monodon indigenous to northern Australia

Mohr, P. G. and Moody, N. J. G. and Hoad, J. and Williams, L. M. and Bowater, R. O. and Cummins, D. M. and Cowley, J. A. and Crane, M. StJ (2015) New yellow head virus genotype (YHV7) in giant tiger shrimp Penaeus monodon indigenous to northern Australia. Diseases of Aquatic Organisms, 115 (3). pp. 263-268.

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Article Link(s): http://dx.doi.org/10.3354/dao02894

Publisher URL: http://www.int-res.com/abstracts/dao/v115/n3/p263-268/

Abstract

ABSTRACT: In 2012, giant tiger shrimp <i>Penaeus monodon</i> originally sourced from Joseph Bonaparte Gulf in northern Australia were examined in an attempt to identify the cause of elevated mortalities among broodstock at a Queensland hatchery. Nucleic acid extracted from ethanol-fixed gills of 3 individual shrimp tested positive using the OIE YHV Protocol 2 RT-PCR designed to differentiate yellow head virus (YHV1) from gill-associated virus (GAV, synonymous with YHV2) and the OIE YHV Protocol 3 RT-nested PCR designed for consensus detection of YHV genotypes. Sequence analysis of the 794 bp (Protocol 2) and 359 bp (Protocol 3) amplicons from 2 distinct regions of ORF1b showed that the yellow-head-complex virus detected was novel when compared with Genotypes 1 to 6. Nucleotide identity on the Protocol 2 and Protocol 3 ORF1b sequences was highest with the highly pathogenic YHV1 genotype (81 and 87%, respectively) that emerged in <i>P. monodon</i> in Thailand and lower with GAV (78 and 82%, respectively) that is enzootic to <i>P. monodon</i> inhabiting eastern Australia. Comparison of a longer (725 bp) ORF1b sequence, spanning the Protocol 3 region and amplified using a modified YH30/31 RT-nPCR, provided further phylogenetic evidence for the virus being distinct from the 6 described YHV genotypes. The virus represents a unique seventh YHV genotype (YHV7). Despite the mortalities observed, the role of YHV7 remains unknown.

Item Type:Article
Business groups:Biosecurity Queensland
Additional Information:10.3354/dao02894
Deposited On:09 Feb 2016 01:34
Last Modified:15 Feb 2016 04:42

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