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Polarized immune responses modulated by layered double hydroxides nanoparticle conjugated with CpG

Yan, Shiyu and Rolfe, Barbara E. and Zhang, Bing and Mohammed, Yousuf H. and Gu, Wenyi and Xu, Zhi P. (2014) Polarized immune responses modulated by layered double hydroxides nanoparticle conjugated with CpG. Biomaterials, 35 (35). pp. 9508-9516. ISSN 0142-9612

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Article Link(s): http://dx.doi.org/10.1016/j.biomaterials.2014.07.0...

Publisher URL: http://www.sciencedirect.com/science/article/pii/S0142961214008643

Abstract

Modulation of the immune response is an important step in the induction of protective humoral and cellular immunity against pathogens. In this study, we investigated the possibility of using a nanomaterial conjugated with the toll-like receptor (TLR) ligand CpG to modulate the immune response towards the preferred polarity. MgAl-layered double hydroxide (LDH) nanomaterial has a very similar chemical composition to Alum, an FDA approved adjuvant for human vaccination. We used a model antigen, ovalbumin (OVA) to demonstrate that MgAl-LDH had comparable adjuvant activity to Alum, but much weaker inflammation. Conjugation of TLR9 ligand CpG to LDH nanoparticles significantly enhanced the antibody response and promoted a switch from Th2 toward Th1 response, demonstrated by a change in the IgG2a:IgG1 ratio. Moreover, immunization of mice with CpG-OVA-conjugated LDH before challenge with OVA-expressing B16/F10 tumor cells retarded tumor growth. Together, these data indicate that LDH nanomaterial can be used as an immune adjuvant to promote Th1 or Th2 dominant immune responses suitable for vaccination purposes.

Item Type:Article
Business groups:Animal Science
Keywords:Layered double hydroxide nanomaterial Nanoadjuvant Th1 and Th2 immune responses Immunity polarization Toll-like receptor (TLR) ligand CpG
Subjects:Veterinary medicine
Deposited On:21 Jan 2015 01:51
Last Modified:21 Jan 2015 01:51

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