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Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre

Guo, S. Y. and Brouwers, H. J. M. and Cobbold, R. N. and Platell, J. L. and Chapman, T. A. and Barrs, V. R. and Johnson, J. R. and Trott, D. J. (2013) Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre. Journal of Antimicrobial Chemotherapy, 68 (5). pp. 1025-1031. ISSN 0305-7453

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Article Link(s): http://dx.doi.org/10.1093/jac/dks515

Abstract

To determine rates of carriage of fluoroquinolone-resistant Escherichia coli and extraintestinal pathogenic E. coli (ExPEC) among dogs in a specialist referral hospital and to examine the population structure of the isolates. Fluoroquinolone-resistant faecal E. coli isolates (n232, from 23 of 123 dogs) recovered from hospitalized dogs in a veterinary referral centre in Sydney, Australia, over 140 days in 2009 were characterized by phylogenetic grouping, virulence genotyping and random amplified polymorphic DNA (RAPD) analysis. The RAPD dendrogram for representative isolates showed one group B2-associated cluster and three group D-associated clusters; each contained isolates with closely related ExPEC-associated virulence profiles. All group B2 faecal isolates represented the O25b-ST131 clonal group and were closely related to recent canine extraintestinal ST131 clinical isolates from the east coast of Australia by RAPD analysis. Hospitalized dogs may carry fluoroquinolone-resistant ExPEC in their faeces, including those representing O25b-ST131.

Item Type:Article
Business groups:Biosecurity Queensland
Additional Information:Guo, SiYu Brouwers, Huub J. M. Cobbold, Rowland N. Platell, Joanne L. Chapman, Toni A. Barrs, Vanessa R. Johnson, James R. Trott, Darren J. University of Queensland Postgraduate Student Scholarship; Australian Research Council; Bayer Health Group [LP0776358]; New South Wales Department of Primary Industries; Office of Research and Development, Medical Research Service, Department of Veterans Affairs [1 I01 CX000192 01]; Merck; Rochester Medical; Syntiron We thank Dr Heinz-Georg Wetzstein for critical reading of the manuscript prior to submission. The assistance with sample collection of Ms Rhonda Foreman and Dr Kenneth Cockwill from the University of Sydney Veterinary Teaching Hospital and assistance with statistics from Idris M. Barchia are gratefully acknowledged. S. Y. G. was the recipient of a University of Queensland Postgraduate Student Scholarship.This study was supported by an Australian Research Council Linkage Project with Bayer Health Group (grant number LP0776358) and the New South Wales Department of Primary Industries. This material is also based on work supported by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs (grant number 1 I01 CX000192 01 to J. R. J.).J. R. J. has received research grants and/or contracts from Merck, Rochester Medical and Syntiron. The other authors declare no potential conflicts of interest. Oxford univ press Oxford
Keywords:E coli ExPEC fluoroquinolone resistance faecal carriage ST131 virulence genotypes companion animals clonal groups phylogeny infection strains humans dna
Subjects:Veterinary medicine > Communicable diseases of animals (General)
Veterinary medicine > Veterinary pathology
Animal culture > Other domesticated and semi-domesticated animals
Deposited On:07 Oct 2014 04:51
Last Modified:07 Oct 2014 04:51

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