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Infection of mungbean seed by Macrophomina phaseolina is more likely to result from localized pod infection than from systemic plant infection

Fuhlbohm, M. J. and Ryley, M. J. and Aitken, E. A. B. (2013) Infection of mungbean seed by Macrophomina phaseolina is more likely to result from localized pod infection than from systemic plant infection. Plant Pathology, 62 (6). pp. 1271-1284. ISSN 0032-0862

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Article Link(s): http://dx.doi.org/10.1111/ppa.12047

Abstract

The ubiquitous fungal pathogen Macrophomina phaseolina is best known as causing charcoal rot and premature death when host plants are subject to post-flowering stress. Overseas reports of M.phaseolina causing a rapid rot during the sprouting of Australian mungbean seed resulted in an investigation of the possible modes of infection of seed. Isolations from serial portions of 10 mungbean plants naturally infected with the pathogen revealed that on most plants there were discrete portions of infected tissue separated by apparently healthy tissue. The results from these studies, together with molecular analysis of isolates collected from infected tissue on two of the plants, suggested that aerial infection of aboveground parts by different isolates is common. Inoculations of roots and aboveground parts of mungbean plants at nine temperaturexsoil moisture incubation combinations and of detached green pods strongly supported the concept that seed infection results from infection of pods by microsclerotia, rather than from hyphae growing systemically through the plant after root or stem infection. This proposal is reinforced by anecdotal evidence that high levels of seed infection are common when rainfall occurs during pod fill, and by the isolation of M.phaseolina from soil peds collected on pods of mungbean plants in the field. However, other experiments showed that when inoculum was placed within 130mm of a green developing pod and a herbicide containing paraquat and diquat was sprayed on the inoculated plants, M.phaseolina was capable of some systemic growth from vegetative tissue into the pods and seeds.

Item Type:Article
Business groups:Crop and Food Science
Additional Information:Fuhlbohm, M. J. Ryley, M. J. Aitken, E. A. B. Grains Research and Development Corporation; Cooperative Research Centre for Tropical Plant Pathology This paper is dedicated to Dr Michael John Fuhlbohm, friend and colleague who died under tragic circumstances on 28 April 2008. The other authors wish to thank the Grains Research and Development Corporation for a Grains Research postgraduate scholarship for Michael, the former Botany Department, University of Queensland and the Cooperative Research Centre for Tropical Plant Pathology for financial and resource support, and Michael's family and friends for their moral support during his PhD studies. Wiley-blackwell Hoboken
Keywords:charcoal rot infection Macrophomina phaseolina mungbean seed survival inoculum residue fungi soil transmission soybeans dna
Subjects:Plant culture > Food crops
Plant pests and diseases > Plant pathology
Deposited On:07 Oct 2014 04:57
Last Modified:07 Oct 2014 04:57

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