Login | Create Account (DAF staff only)

Variable-number tandem repeats genotyping used to aid and inform management strategies for a bovine Johne's disease incursion in tropical and subtropical Australia

Oakey, J. and Gavey, L. and Singh, S. V. and Platell, J. and Waltisbuhl, D. (2014) Variable-number tandem repeats genotyping used to aid and inform management strategies for a bovine Johne's disease incursion in tropical and subtropical Australia. Journal of Veterinary Diagnostic Investigation . ISSN 1040-6387

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link(s): http://dx.doi.org/10.1177/1040638714547257

Abstract

The application of variable-number tandem repeats (VNTR) genotyping of Mycobacterium avium subsp. paratuberculosis isolates to assist in investigating incidents of bovine Johne’s disease in a low-prevalence region of Australia is described in the current study. Isolates from a response to detection of bovine Johne’s disease in Queensland were compared with strains from national and international sources. The tandem application of mycobacterial interspersed repetitive unit (MIRU) and multilocus short sequence repeats (MLSSR) genotyping identified 2 strains, 1 that infected cattle on multiple properties with trace-forward histories from a common infected property, and 1 genotypically different strain recovered from a single property. The former strain showed an identical genotype to an isolate from India. Neither strain showed a genotypic link to regions of Australia with a higher prevalence of the disease. Genotyping has indicated incursions from 2 independent sources. This intelligence has informed investigations into potential routes of entry and the soundness of ongoing control measures, and supported strategy and policy decisions regarding management of Mycobacterium avium subsp. paratuberculosis incursions for Queensland.

Item Type:Article
Business groups:Biosecurity Queensland
Subjects:Animal culture > Cattle
Veterinary medicine > Veterinary pathology
Veterinary medicine > Diseases of special classes of animals > Cattle
Deposited On:27 Aug 2014 00:11
Last Modified:27 Aug 2014 00:11

Repository Staff Only: item control page